2C4-Gamma2A cell line
Invented by George Stark at Cancer Research UK London Research Institute: Lincoln's Inn Fields Cleveland Clinic Foundation
Gamma2A Jak2 Cell Line
Invented by George Stark , Ian Kerr
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (6)
- Inventor Info
Info
| Catalogue Number | 151681 |
| Antigen/Gene or Protein Targets | Gamma2A Jak2 |
| Parental Line | HT 1080 |
| Host | Human |
| Disease Keywords | Fibrosarcoma |
| Model | Transgenic |
| Relevance | The Gamma2A Jak2 Cell Line has been developed by expressing protein tyrosine kinase JAK2 in a mutant cell line defective in the interferon-gamma signal transduction pathway. By using this cell line, the structural and functional analysis of JAk2 in IFN-gamma signalling has been examined. The cell line revealed that the specificity of JAK may lie mainly in their structural interaction with different receptors and signalling proteins rather than in the substrate specificity of their kinase domains. |
| Production Details | The human sarcoma cell line HT 1080 was transfected with a vector encoding a selectable marker (guanine phosphoribosyltransferase) regulated by gamma interferon to create the parent 2C4- gamma 2A cell line. This cell line was then co-transfected with expression construct coding for JAK2. |
| Research Area | Cell Signaling & Signal Transduction, Immunology |
| Recommended Growing Conditions | DMEM with 10% v/v heat-inactivated FCS and 400ug/ml G418 plus 0.5 ug/ml puromycin |
| Cellosaurus ID | CVCL_1D51 |
2C4 cell line
Invented by George Stark at Cleveland Clinic Foundation Cancer Research UK London Research Institute: Lincoln's Inn Fields
References: 6 entries
Bonjardim et al. 1998. Braz J Med Biol Res. 31(11):1389-95. PMID: 9921273.
JAK/STAT-deficient cell lines.
Europe PMC ID: 9921273
Kohlhuber et al. 1997. Mol Cell Biol. 17(2):695-706. PMID: 9001223.
A JAK1/JAK2 chimera can sustain alpha and gamma interferon responses.
Europe PMC ID: 9001223
Watling et al. 1993. Nature. 366(6451):166-70. PMID: 7901766.
Complementation by the protein tyrosine kinase JAK2 of a mutant cell line defective in the interferon-gamma signal transduction pathway.
Europe PMC ID: 7901766
Add a reference
References: 6 entries
Bonjardim et al. 1998. Braz J Med Biol Res. 31(11):1389-95. PMID: 9921273.
JAK/STAT-deficient cell lines.
Kohlhuber et al. 1997. Mol Cell Biol. 17(2):695-706. PMID: 9001223.
A JAK1/JAK2 chimera can sustain alpha and gamma interferon responses.
Watling et al. 1993. Nature. 366(6451):166-70. PMID: 7901766.
Complementation by the protein tyrosine kinase JAK2 of a mutant cell line defective in the interferon-gamma signal transduction pathway.
Add a reference
Inventor Information
Inventors
|
George Stark |
|
|
Ian Kerr |
