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Monoclonal Antibodies

Anti-Metapneumovirus Phosphoprotein [hMPV123]

Invented at University of Newcastle upon Tyne
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Image thumbnail for Anti-Metapneumovirus Phosphoprotein [hMPV123]
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Adapted from Tedcastle et al. 2012. J Med Virol. 84(7):1061-70. PMID: 22585723. Figure. Western blots of HMPV strain NCL174 passaged 14 times in 16HBE140 cells (lanes 1, 3, and 5) and uninfected 16HBE140 cells (lanes 2, 4, and 6) stained with rabbit polyclonal antiserum to HMPV (lanes 1,2), MAb57 (lanes 3,4; pattern 1) and MAb123 (lanes 5,6; pattern 2). The numbers to the right of the figure indicate the molecular weights (kDa) of the major bands calculated from the standard curve generated from the migration of molecular weight markers in the same gel.
Adapted from Cifuentes-Muñoz et al. 2017. J Virol; 91(24). PMID: 28978704. Figure. BEAS-2B cells were infected with HMPV CAN97-83 at an MOI of 3 PFU/cell and fixed at 24 hpi. FISH and immunofluorescence were performed to detect vRNA (red), +RNA (purple; green as a pseudocolor), polyadenylated RNAs (red), and the HMPV P protein (hMPV123} (green). Bars, 10 μm. The squares with dotted outlines are magnified and shown as the zoom images in the right column.
Adapted from El Najjar et al. 2016. PLoS Pathog. 12(9):e1005922. PMID: 27683250. Figure. HMPV infected BEAS-2B cells at 48 h.p.i. were fixed, processed for immunofluorescence and stained with antibodies for HMPV N or P (hMPV123). Scale bar = 50 μm.
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Catalogue Number 151643
Applications ELISA IHC IF WB
Antigen/Gene or Protein Targets Metapneumovirus Phosphoprotein
Reactivity Virus
Relevance Identification and location of expression of the phosphoprotein of human metapneumovirus (hMPV) of both types A and B.
Host Mouse
Immunogen Human MPV strain NCL-145
Positive Control Human MPV infected LLC-MK2 cells
Subclass IgG2a
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI + Hepes (25mM) + glutamine (2mM) + 10% heat inactivated FCS.
Notes ELISA 1:100-1:2000

Reactivity: Metapneumovirus (MPV)
Research Area Virology

References

There are 6 reference entries for this reagent.

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References: 6 entries

Cifuentes-Muñoz et al. 2017. J Virol. 91(24):. PMID: 28978704.

IF

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El Najjar et al. 2016. PLoS Pathog. 12(9):e1005922. PMID: 27683250.

IF

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Tedcastle et al. 2012. J Med Virol. 84(7):1061-70. PMID: 22585723.

WB

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The characterization of monoclonal antibodies to human metapneumovirus and the detection of multiple forms of the virus nucleoprotein and phosphoprotein.

Europe PMC ID: 22585723

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Fenwick et al. 2007. J Clin Virol. 40(3):193-6. PMID: 17869169.

IF

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Diagnosis of human metapneumovirus by immunofluorescence staining with monoclonal antibodies in the North-East of England.

Europe PMC ID: 17869169

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References: 6 entries

Cifuentes-Muñoz et al. 2017. J Virol. 91(24):. PMID: 28978704.

IF

View  

El Najjar et al. 2016. PLoS Pathog. 12(9):e1005922. PMID: 27683250.

IF

View  

Tedcastle et al. 2012. J Med Virol. 84(7):1061-70. PMID: 22585723.

WB

View  

The characterization of monoclonal antibodies to human metapneumovirus and the detection of multiple forms of the virus nucleoprotein and phosphoprotein.

View  

Fenwick et al. 2007. J Clin Virol. 40(3):193-6. PMID: 17869169.

IF

View  

Diagnosis of human metapneumovirus by immunofluorescence staining with monoclonal antibodies in the North-East of England.

View  

Add a reference

Inventor Information

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Invented at

University of Newcastle upon Tyne
University of Newcastle upon Tyne

39 reagents listed on Ximbio