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Anti-Metapneumovirus Fusion Protein [hMPV24]

Info

Catalogue Number 151640
Applications ELISA IHC IF
Antigen/Gene or Protein Targets Metapneumovirus Fusion Protein
Reactivity Virus
Relevance Monoclonal antibody which can identify human metapneumovirus via immunofluorescent staining and does not cross react to other cell cultures

Background and Research Application
Anti-hMPV monoclonal antibody which can identify and locate the fusion glycoprotein of human metapneumovirus (hMPV) of both types A and B, within a sample.
hMPV is a negative single stranded RNA virus of the paramyxoviridae family and can cause upper and lower respiratory tract infections. Discovered in 2001, there appear to be 4 lineages of the hMPV virus, stemming from sub-groups A and B.Anti-hMPV24 can recognise the fusion protein of all 4 lineages(A1,A2, B1 and B2). Anti-hMPV24 be also used in conjunction with other hMPV antibody clones to detect all strains of the virus.
Anti-hMPV24 was created to diagnose hMPV infection in respiratory specimens via immunofluorescence staining with optimal staining of clinical samples with low backgrounds on uninfected specimens.
Host Mouse
Immunogen Human MPV strain NCL-145
Positive Control Human MPV infected LLC-MK2 cells
Subclass IgG2a
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI + Hepes (25mM) + glutamine (2mM) + 10% heat inactivated FCS.
Strain Balb/c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Recommended concentration for immunofluorescence: 20ug/ml

Reactivity: Metapneumovirus (MPV)
Anti-hMPV24 does not react with cell cultures infected with hRSV, influenzavirus A or B, adenovirus, parainfluenza viruses 1-4, mumps virus, measles virus, varicella-zoster virus, herpes simplex virus types 1 and 2, human cytomegalovirus, human herpes virus type 6, ECHOvirus 19, Coxsachievirus B4, poliovirus types1–3. HHV6 and against uninfected HeLa, MA104, 3MK, LLC-MK, HEp2, MRC-5 and HSB-2 cells

Concentration
2mg/ml
Research Area Virology

References

There are 4 reference entries for this reagent.

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References: 4 entries

Tedcastle et al. 2012. J Med Virol. 84(7):1061-70. PMID: 22585723.

IF

The characterization of monoclonal antibodies to human metapneumovirus and the detection of multiple forms of the virus nucleoprotein and phosphoprotein.

Europe PMC ID: 22585723

Fenwick et al. 2007. J Clin Virol. 40(3):193-6. PMID: 17869169.

IF

Diagnosis of human metapneumovirus by immunofluorescence staining with monoclonal antibodies in the North-East of England.

Europe PMC ID: 17869169


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References: 4 entries

Tedcastle et al. 2012. J Med Virol. 84(7):1061-70. PMID: 22585723.

IF

The characterization of monoclonal antibodies to human metapneumovirus and the detection of multiple forms of the virus nucleoprotein and phosphoprotein.

Fenwick et al. 2007. J Clin Virol. 40(3):193-6. PMID: 17869169.

IF

Diagnosis of human metapneumovirus by immunofluorescence staining with monoclonal antibodies in the North-East of England.


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Inventor Information

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