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Polyclonal Antibodies

Anti-WSTF, Polyclonal [WSTF]

Invented at Marie Curie Research Institute
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Adapted from Sarshad et al. 2013. PLoS Genet. 9(3):e1003397. PMID: 23555303. Figure. During cell division, WSTF phosphorylation events govern the assembly and disassembly of B-WICH. (A) NM1, SNF2h and WSTF are co-precipitated from protein extracts prepared from growing HeLa cells. Bound proteins were detected on immunoblots. 10% of the input is shown in Lane 1. IP, immunoprecipitation. (B) Co-precipitations of NM1, SNF2h and WSTF are impaired from protein extracts prepared from mitotic HeLa cells at t = 0 min from the nocodazole block. Bound proteins were detected on immunoblots. 10% of the input is loaded in Lane 1. IP, immunoprecipitation. (C) WSTF becomes phosphorylated at the onset of mitosis. Lysates were prepared from growing HeLa cells (Lane 1), mitotic HeLa cells at t = 0 min from the nocodazole block (Lanes 2 and 3) or HeLa cells after t = 120 min release from the block (Lanes 4 and 5). Where indicated extracts were subjected to phosphatase treatment (Lanes 3 and 5). In all cases lysates were separated by 7% phospho-affinity SDS-PAGE and analyzed on immunoblots for WSTF and actin. (D) Co-precipitations of WSTF, SNF2h and NM1 are dependent on WSTF phosphorylation. Mitotic extracts from HeLa cells at t = 0 min from the nocodazole block, untreated (Lanes 1–3) or treated with phosphatase (Lanes 4–6), were subjected to immunoprecipitations with anti-WSTF antibodies or non-specific IgGs. Bound proteins were separated by SDS PAGE and analyzed on immunoblots for WSTF, SNF2h and NM1. 5% of the input is shown in Lane 1. IP, immunoprecipitation. (E–F) WSTF steady state expression levels on immunoblots of lysates prepared from control (scrRNAi) and WSTF-silenced HeLa cells. Right panel, densitometric quantification of WSTF steady state protein expression relative to actin. (G) In growing HeLa cells, co-precipitations of NM1 and SNF2h are impaired as a consequence of WSTF gene knockdown by siRNA (WSTF RNAi) but not when cells are subjected to control experiments with scrambled RNAi oligonucleotides (scrRNAi). In Lanes 1 and 4, 20% of the input material was loaded.
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Catalogue Number 151586
Applications IF IP WB
Antigen/Gene or Protein Targets Williams Syndrome Transcription Factor (WSTF)
Reactivity Human and Mouse
Relevance The human Williams Syndrome Transcription Factor (WSTF) gene is located within the common Williams Syndrome deletion area at chromosome 7q11.23. Its functional motifs include a PHD zinc finger motif followed by a bromodomain. Both motifs are found in many transcription factors, suggesting that WSTF may function as a transcription factor. WSTF forms a chromatinremodelling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The drosophila Acf1 gene is structurally related to the human WSTF gene.
Host Rabbit
Immunogen Peptide coupled to keyhole limpet hemocyanin.
Formulation Whole serum
Molecular Weight (kDa) 170
Notes Suggested dilutions for western blot: 1:2000
Positive Control ES, NIH 3T3, and HeLa cells
Research Area Epigenetics & Nuclear Signalling

References

There are 4 reference entries for this reagent.

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References: 4 entries

Sarshad et al. 2013. PLoS Genet. 9(3):e1003397. PMID: 23555303.

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Nuclear myosin 1c facilitates the chromatin modifications required to activate rRNA gene transcription and cell cycle progression.

Europe PMC ID: 23555303

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Bozhenok et al. 2002. EMBO J. 21(9):2231-41. PMID: 11980720.

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WSTF-ISWI chromatin remodeling complex targets heterochromatic replication foci.

Europe PMC ID: 11980720

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References: 4 entries

Sarshad et al. 2013. PLoS Genet. 9(3):e1003397. PMID: 23555303.

View  

Nuclear myosin 1c facilitates the chromatin modifications required to activate rRNA gene transcription and cell cycle progression.

View  

Bozhenok et al. 2002. EMBO J. 21(9):2231-41. PMID: 11980720.

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WSTF-ISWI chromatin remodeling complex targets heterochromatic replication foci.

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Inventor Information

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Invented at

Marie Curie Research Institute
Marie Curie Research Institute

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