- References (2)
- Inventor Info
|Applications||FACS IF IP WB|
|Antigen/Gene or Protein Targets||Ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1, CD39)|
Monoclonal antibody which binds ENTPD1, and reacts with Epstein Barr lymphoblastoid cell lines.
Background and Research Application
ENTPD1 is a transmembrane glycoprotein of apparent molecular weight of 70-100 kD that has an Ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1, ecto-apyrase) activity hydrolysing the alpha- and beta- phosphate residues of extracellular ATP or ADP. In haematopoietic tissues, it is primarily expressed on activated B, T and NK cells. It is also expressed on some endothelial cells and cardiac neurons, and helps regulate platelet activation, vascular inflammation, and cardiac norepinephrine release.
ACE2 reacted consistently with all Epstein Barr virus lymphoblastoid cell line (EB‐LCL) tested, with a subpopulation of cells in some but not all EB virus genome‐positive Burkitt lymphoma lines, but with none of a range of EB virus genome‐negative cell lines of lymphoma or leukaemia origin.
|Immunogen||EBV-transformed human B lymphoblastoid cell line|
|Immunogen UniProt ID||O75356|
|Positive Control||EBV-transformed human B lymphoblastoid cell line|
|Molecular Weight (kDa)||70-100|
Purified using multi-step affinity chromatography with protein A.
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.
Points of Interest
ACE2 was unable to protect EB‐LCL target cells from lysis by LYDMA‐specific cytotoxic T cells in a chromium‐release assay.
FACS: 1µg/5x105 cells
Immunofluorescence on acetone-fixed frozen sections or cell smears: 1-2µg/ml
Western blot (ECL): 0.2µg/ml
1mg/ml as standard
|Research Area||Cardiovascular, Cell Type or Organelle Marker, Immunology, Neurobiology|
There are 2 reference entries for this reagent.View All References
References: 2 entries
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