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Anti-ENTPD1 [AC2]

Invented by Prof Martin Rowe at University Of Birmingham

Info

Catalogue Number 151580
Applications FACS IF IP WB
Antigen/Gene or Protein Targets Ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1, CD39)
Reactivity Human
Relevance Monoclonal antibody which binds ENTPD1, and reacts with Epstein Barr lymphoblastoid cell lines.

Background and Research Application
ENTPD1 is a transmembrane glycoprotein of apparent molecular weight of 70-100 kD that has an Ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1, ecto-apyrase) activity hydrolysing the alpha- and beta- phosphate residues of extracellular ATP or ADP. In haematopoietic tissues, it is primarily expressed on activated B, T and NK cells. It is also expressed on some endothelial cells and cardiac neurons, and helps regulate platelet activation, vascular inflammation, and cardiac norepinephrine release.

ACE2 reacted consistently with all Epstein Barr virus lymphoblastoid cell line (EB‐LCL) tested, with a subpopulation of cells in some but not all EB virus genome‐positive Burkitt lymphoma lines, but with none of a range of EB virus genome‐negative cell lines of lymphoma or leukaemia origin.
Host Mouse
Immunogen EBV-transformed human B lymphoblastoid cell line
Immunogen UniProt ID O75356
Positive Control EBV-transformed human B lymphoblastoid cell line
Subclass IgG1
Molecular Weight (kDa) 70-100
Myeloma Used P3X63Ag8.653
Strain Balb/c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
ACE2 was unable to protect EB‐LCL target cells from lysis by LYDMA‐specific cytotoxic T cells in a chromium‐release assay.

Recommended Concentrations:

FACS: 1µg/5x105 cells

Immunofluorescence on acetone-fixed frozen sections or cell smears: 1-2µg/ml

Western blot (ECL): 0.2µg/ml

Concentration
1mg/ml as standard
Research Area Cardiovascular, Cell Type or Organelle Marker, Immunology, Neurobiology

References

There are 2 reference entries for this reagent.

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References: 2 entries

Rowe et al. 1982. Int J Cancer. 29(4):373-81. PMID: 6282762.

Monoclonal antibodies to Epstein-Barr virus-induced, transformation-associated cell surface antigens: binding patterns and effect upon virus-specific T-cell cytotoxicity.

Europe PMC ID: 6282762


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References: 2 entries

Rowe et al. 1982. Int J Cancer. 29(4):373-81. PMID: 6282762.

Monoclonal antibodies to Epstein-Barr virus-induced, transformation-associated cell surface antigens: binding patterns and effect upon virus-specific T-cell cytotoxicity.


Add a reference