Cat. #151580
Anti-ENTPD1 [AC2]
Cat. #: 151580
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-5 days
Target: Ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1, CD39)
Class: Monoclonal
Application: FACS ; IF ; IP ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Martin Rowe
Institute: University of Birmingham
Primary Citation: Rowe et al. 1982. Int J Cancer. 29(4):373-81. PMID: 6282762.
Tool Details
*FOR RESEARCH USE ONLY
- Name: Anti-ENTPD1 [AC2]
- Clone: AC2
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 70-100 kDa
- Strain: Balb/c
- Reactivity: Human
- Host: Mouse
- Application: FACS ; IF ; IP ; WB
- Description: Monoclonal antibody which binds ENTPD1, and reacts with Epstein Barr lymphoblastoid cell lines. ACE2 reacted consistently with all Epstein Barr virus lymphoblastoid cell line (EB-LCL) tested, with a subpopulation of cells in some but not all EB virus genome-positive Burkitt lymphoma lines, but with none of a range of EB virus genome-negative cell lines of lymphoma or leukaemia origin.
- Immunogen: EBV-transformed human B lymphoblastoid cell line
- Immunogen uniprot id: O75356
- Isotype: IgG1
- Myeloma used: P3X63Ag8.653
- Recommended controls: EBV-transformed human B lymphoblastoid cell line as positive control
Target Details
- Target: Ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1, CD39)
- Molecular weight: 70-100 kDa
- Tissue cell line specificity: EBV-transformed human B lymphoblastoid cell line
- Target background: ENTPD1 is a transmembrane glycoprotein of apparent molecular weight of 70-100 kD that has an Ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1, ecto-apyrase) activity hydrolysing the alpha- and beta- phosphate residues of extracellular ATP or ADP. In haematopoietic tissues, it is primarily expressed on activated B, T and NK cells. It is also expressed on some endothelial cells and cardiac neurons, and helps regulate platelet activation, vascular inflammation, and cardiac norepinephrine release.
Applications
- Application: FACS ; IF ; IP ; WB
- Application notes: Recommended Concentrations:
FACS: 1ug/5x10^5 cells
Immunofluorescence on acetone-fixed frozen sections or cell smears: 1-2ug/ml
Western blot (ECL): 0.2ug/ml
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4° C
References
- Rowe et al. 1982. Int J Cancer. 29(4):373-81. PMID: 6282762.
