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Anti-CD44v4/5 [3D2]

Info

Catalogue Number 151574
Applications FACS IHC IP WB
Antigen/Gene or Protein Targets CD44v3-10-Fc
Synonyms CD44 Molecule; Hematopoietic Cell E- And L-Selectin Ligand; GP90 Lymphocyte Homing/Adhesion Receptor; Chondroitin Sulfate Proteoglycan 8; Extracellular Matrix Receptor III; Heparan Sulfate Proteoglycan; Phagocytic Glycoprotein; Hyaluronate Receptor; Hermes Antigen; ECMR-III; HUTCH-I; Epican; CDW44; MDU2; MDU3; MIC4; LHR; Cell Surface Glycoprotein CD44; Phagocytic Glycoprotein I; Soluble CD44; CSPG8; HCELL; PGP-1; MC56; Pgp1
Reactivity Human
Relevance Monoclonal antibody directed against CD44v4/5, strongly expressed on metastatic breast cancer cells.

Background and Research Application
CD44 is an integral cell membrane glycoprotein that binds to hyaluronan and is involved in matrix adhesion, lymphocyte activation, and lymph node homing. The CD44 protein is expressed as a family of molecular isoforms generated from alternative RNA splicing and posttranslational modifications. CD44 is expressed on multiple cell types and is involved in multiple functions including cell-cell interactions and cell-extracellular matrix binding. CD44 participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, haematopoiesis, and tumour metastasis. Hyaluronan, a high molecular weight polysaccharide component of the extracellular matrix acts as the principal ligand for the CD44 receptor.
Human CD44v4/5 is specific for CD44 protein isoforms containing human variant exons 4 and 5. Metastatic breast cancer cells strongly express a ∼170 kD CD44 variant 4 (CD44v4).
Host Mouse
Immunogen CD44v3-10-Fc Human chimeric fusion protein
Immunogen UniProt ID P16070
Subclass IgG1
Myeloma Used Sp2/0-Ag14
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Western Blot 1 µg/mL
Flow Cytometry 2.5 µg/106 cells
Immunocytochemistry 8-25 µg/mL

Concentration
1mg/ml as standard
Research Area Adhesion, Cancer, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Immunology

References

There are 4 reference entries for this reagent.

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References: 4 entries

Zen et al. 2008. PLoS One. 3(3):e1826. PMID: 18350162.

Assimakopoulos et al. 2002. Histol Histopathol. 17(4):1269-81. PMID: 12371152.

Fox et al. 1994. Cancer Res. 54(16):4539-46. PMID: 7519124.

Normal human tissues, in addition to some tumors, express multiple different CD44 isoforms.

Europe PMC ID: 7519124


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References: 4 entries

Zen et al. 2008. PLoS One. 3(3):e1826. PMID: 18350162.

Assimakopoulos et al. 2002. Histol Histopathol. 17(4):1269-81. PMID: 12371152.

Fox et al. 1994. Cancer Res. 54(16):4539-46. PMID: 7519124.

Normal human tissues, in addition to some tumors, express multiple different CD44 isoforms.


Add a reference

Inventor Information

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