Anti-CD44v4/5 [3D2]
Invented at University of Oxford
- Datasheet
- References (4)
- Inventor Info
Info
| Catalogue Number | 151574 |
| Applications | FACS IHC IP WB |
| Antigen/Gene or Protein Targets | CD44v3-10-Fc |
| Synonyms | CD44 Molecule; Hematopoietic Cell E- And L-Selectin Ligand; GP90 Lymphocyte Homing/Adhesion Receptor; Chondroitin Sulfate Proteoglycan 8; Extracellular Matrix Receptor III; Heparan Sulfate Proteoglycan; Phagocytic Glycoprotein; Hyaluronate Receptor; Hermes Antigen; ECMR-III; HUTCH-I; Epican; CDW44; MDU2; MDU3; MIC4; LHR; Cell Surface Glycoprotein CD44; Phagocytic Glycoprotein I; Soluble CD44; CSPG8; HCELL; PGP-1; MC56; Pgp1 |
| Reactivity | Human |
| Relevance |
Monoclonal antibody directed against CD44v4/5, strongly expressed on metastatic breast cancer cells. Background and Research Application CD44 is an integral cell membrane glycoprotein that binds to hyaluronan and is involved in matrix adhesion, lymphocyte activation, and lymph node homing. The CD44 protein is expressed as a family of molecular isoforms generated from alternative RNA splicing and posttranslational modifications. CD44 is expressed on multiple cell types and is involved in multiple functions including cell-cell interactions and cell-extracellular matrix binding. CD44 participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, haematopoiesis, and tumour metastasis. Hyaluronan, a high molecular weight polysaccharide component of the extracellular matrix acts as the principal ligand for the CD44 receptor. Human CD44v4/5 is specific for CD44 protein isoforms containing human variant exons 4 and 5. Metastatic breast cancer cells strongly express a ∼170 kD CD44 variant 4 (CD44v4). |
| Host | Mouse |
| Immunogen | CD44v3-10-Fc Human chimeric fusion protein |
| Immunogen UniProt ID | P16070 |
| Subclass | IgG1 |
| Myeloma Used | Sp2/0-Ag14 |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest Western Blot 1 µg/mL Flow Cytometry 2.5 µg/106 cells Immunocytochemistry 8-25 µg/mL Concentration 1mg/ml as standard |
| Research Area | Adhesion, Cancer, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Immunology |
References: 4 entries
Zen et al. 2008. PLoS One. 3(3):e1826. PMID: 18350162.
Assimakopoulos et al. 2002. Histol Histopathol. 17(4):1269-81. PMID: 12371152.
Fox et al. 1994. Cancer Res. 54(16):4539-46. PMID: 7519124.
Normal human tissues, in addition to some tumors, express multiple different CD44 isoforms.
Europe PMC ID: 7519124
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References: 4 entries
Zen et al. 2008. PLoS One. 3(3):e1826. PMID: 18350162.
Assimakopoulos et al. 2002. Histol Histopathol. 17(4):1269-81. PMID: 12371152.
Fox et al. 1994. Cancer Res. 54(16):4539-46. PMID: 7519124.
Normal human tissues, in addition to some tumors, express multiple different CD44 isoforms.
Add a reference
