Cat. #151574
Anti-CD44v4/5 [3D2]
Cat. #: 151574
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 1-2 weeks
Target: CD44v3-10-Fc
Class: Monoclonal
Application: FACS ; IHC ; IP ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: David Simmons
Institute: University of Oxford
Tool Details
*FOR RESEARCH USE ONLY
- Name: Anti-CD44v4/5 [3D2]
- Alternate name: CD44 Molecule; Hematopoietic Cell E- And L-Selectin Ligand; GP9 Lymphocyte Homing/Adhesion Receptor; Chondroitin Sulfate Proteoglycan 8; Extracellular Matrix Receptor III; Heparan Sulfate Proteoglycan; Phagocytic Glycoprotein; Hyaluronate Receptor; Hermes Antigen; ECMR-III; HUTCH-I; Epican; CDW44; MDU2; MDU3; MIC4; LHR; Cell Surface Glycoprotein CD44; Phagocytic Glycoprotein I; Soluble CD44; CSPG8; HCELL; PGP-1; MC56; Pgp1
- Clone: 3D2
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Reactivity: Human
- Host: Mouse
- Application: FACS ; IHC ; IP ; WB
- Description: Monoclonal antibody directed against CD44v4/5, strongly expressed on metastatic breast cancer cells.
- Immunogen: CD44v3-10-Fc Human chimeric fusion protein
- Immunogen uniprot id: P16070
- Isotype: IgG1
- Myeloma used: Sp2/0-Ag14
Target Details
- Target: CD44v3-10-Fc
- Target background: Monoclonal antibody directed against CD44v4/5, strongly expressed on metastatic breast cancer cells. Background and Research Application CD44 is an integral cell membrane glycoprotein that binds to hyaluronan and is involved in matrix adhesion, lymphocyte activation, and lymph node homing. The CD44 protein is expressed as a family of molecular isoforms generated from alternative RNA splicing and posttranslational modifications. CD44 is expressed on multiple cell types and is involved in multiple functions including cell-cell interactions and cell-extracellular matrix binding. CD44 participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, haematopoiesis, and tumour metastasis. Hyaluronan, a high molecular weight polysaccharide component of the extracellular matrix acts as the principal ligand for the CD44 receptor. Human CD44v4/5 is specific for CD44 protein isoforms containing human variant exons 4 and 5. Metastatic breast cancer cells strongly express a ÄËĂÂ?170 kD CD44 variant 4 (CD44v4).
Applications
- Application: FACS ; IHC ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
- Shipping conditions: Shipping at 4° C
References
- Zen et al. 2008. PLoS One. 3(3):e1826. PMID: 18350162.
- Assimakopoulos et al. 2002. Histol Histopathol. 17(4):1269-81. PMID: 12371152.
- Fox et al. 1994. Cancer Res. 54(16):4539-46. PMID: 7519124.
- Normal human tissues, in addition to some tumors, express multiple different CD44 isoforms.
