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Monoclonal Antibodies

Anti-Zebrafish gut absorptive cell epitopes [FIS 4E8/1]

Invented by Linda Ariza-McNaughton from Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
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Image thumbnail for Anti-Zebrafish gut absorptive cell epitopes [FIS 4E8/1]
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Adapted from Matthews et al. 2008. Dev Dyn. 237(1):124-31. PMID: 18095340. Figure. Confocal projections of 2F11 immunostained 5-dpf whole-mount livers from control (H) and Oc3 morpholino-injected (I) larvae. J, K: Schematic representation of the confocal projections in H and I.
Adapted from Dong et al. 2007. Nat Genet; 39(3):397-402. PMID: 17259985. Figure (a,b) Three-dimensional rendering of the hepatopancreatic system showing 80-hpf Tg(elastase:GFP); Tg(lfabp:dsRed) larva labeled with monoclonal antibody 2F11 (blue) to mark hepatopancreatic ductal system and somatostatin (Sst) to mark the principal islet of the pancreas (P).(c) Illustration of hepatopancreatic ductal system (extrapancreatic duct, EPD red; hepatopancreatic ampulla, HPA, green; common bile duct, CBD, blue; cystic duct, CD, orange; extrahepatic duct, EHD, yellow).
Adapted from Dong et al. 2007. Nat Genet. 39(3):397-402. PMID: 17259985. Figure. Three-dimensional rendering of the hepatopancreatic ductal system marked by 2F11.
Adapted from Crosnier et al. 2005. Development. 132(5):1093-104. PMID: 15689380. Figure (A) Western blot of zebrafish gut lysate resolved under non-reducing conditions on SDS-PAGE; antibodies 2F11, 4B7/1 and 3G12 have different antigen specificities. (B) 2F11 (green) stain differentiated cells in the intestinal bulb of a 5-day-old larva. (C-H) 2F11 immunolabelling.
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Catalogue Number 151512
Applications IHC IP WB
Antigen/Gene or Protein Targets Zebrafish gut absorptive cell epitopes
Reactivity Zebrafish
Relevance The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. This antibody labels the brush border / glycocalyxof intestinal absorptive cells (apical ends of microvilli). This antibody is not reactive with goblet cells; thus 4E8 can be used as a marker for brush border cells.
Host Mouse
Immunogen Lysate of zebrafish intestines
Subclass IgG1
Molecular Weight (kDa) 200
Myeloma Used Sp2/0-Ag14
Strain Balb/c
Notes Suggested Dilution: 1:200

4E8/1 may be used on formalin fixed gut sections of zebrafish.

Alternative name: 2F11 antibody
Research Area Aquaculture, Cell Cycle, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Developmental Biology, Epigenetics & Nuclear Signalling, Epitope Tags, Stem Cell Biology

References

There are 8 reference entries for this reagent.

View All References

References: 8 entries

Matthews et al. 2008. Dev Dyn. 237(1):124-31. PMID: 18095340.

IHC

View  

Transcription factor onecut3 regulates intrahepatic biliary development in zebrafish.

Europe PMC ID: 18095340

View  

Dong et al. 2007. Nat Genet. 39(3):397-402. PMID: 17259985.

IHC

View  

Fgf10 regulates hepatopancreatic ductal system patterning and differentiation.

Europe PMC ID: 17259985

View  

Bates et al. 2006. Dev Biol. 297(2):374-86. PMID: 16781702.

View  

Distinct signals from the microbiota promote different aspects of zebrafish gut differentiation.

Europe PMC ID: 16781702

View  

Crosnier et al. 2005. Development. 132(5):1093-104. PMID: 15689380.

IHC WB

View  

Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine.

Europe PMC ID: 15689380

View  

Add a reference

References: 8 entries

Matthews et al. 2008. Dev Dyn. 237(1):124-31. PMID: 18095340.

IHC

View  

Transcription factor onecut3 regulates intrahepatic biliary development in zebrafish.

View  

Dong et al. 2007. Nat Genet. 39(3):397-402. PMID: 17259985.

IHC

View  

Fgf10 regulates hepatopancreatic ductal system patterning and differentiation.

View  

Bates et al. 2006. Dev Biol. 297(2):374-86. PMID: 16781702.

View  

Distinct signals from the microbiota promote different aspects of zebrafish gut differentiation.

View  

Crosnier et al. 2005. Development. 132(5):1093-104. PMID: 15689380.

IHC WB

View  

Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine.

View  

Add a reference

Inventor Information