#151512

Anti-Zebrafish gut absorptive cell epitopes [FIS 4E8/1]

Cat. #151512

Anti-Zebrafish gut absorptive cell epitopes [FIS 4E8/1]

Cat. #: 151512

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 1-2 weeks

Target: Zebrafish gut absorptive cell epitopes

Class: Monoclonal

Application: IHC ; IP ; WB

Reactivity: Zebrafish

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Linda Ariza-McNaughton

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Target Details
Applications
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-Zebrafish gut absorptive cell epitopes [FIS 4E8/1]
  • Clone: FIS 4E8/1; 2F11
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Molecular weight: 200 kDa
  • Strain: Balb/c
  • Reactivity: Zebrafish
  • Host: Mouse
  • Application: IHC ; IP ; WB
  • Description: This antibody is not reactive with goblet cells; thus 4E8 can be used as a marker for brush border cells.
  • Immunogen: Lysate of zebrafish intestines
  • Isotype: IgG1
  • Myeloma used: Sp2/0-Ag14

Target Details

  • Target: Zebrafish gut absorptive cell epitopes
  • Molecular weight: 200 kDa
  • Target background: The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. This antibody labels the brush border / glycocalyxof intestinal absorptive cells (apical ends of microvilli).

Applications

  • Application: IHC ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15° C to -25° C
  • Shipping conditions: Shipping at 4° C

Related Tools

  • Related tools: Anti-Zebrafish Basolateral Pole of Cells [FIS 2H9/1] ; Anti-Zebrafish Basolateral Pole of Cells [FIS 2H9/1]

References

  • Matthews et al. 2008. Dev Dyn. 237(1):124-31. PMID: 18095340.
  • Transcription factor onecut3 regulates intrahepatic biliary development in zebrafish.
  • Dong et al. 2007. Nat Genet. 39(3):397-402. PMID: 17259985.
  • Fgf10 regulates hepatopancreatic ductal system patterning and differentiation.
  • Bates et al. 2006. Dev Biol. 297(2):374-86. PMID: 16781702.
  • Distinct signals from the microbiota promote different aspects of zebrafish gut differentiation.
  • Crosnier et al. 2005. Development. 132(5):1093-104. PMID: 15689380.
  • Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine.