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Monoclonal Antibodies

Anti-Zebrafish gut secretory cell epitopes [FIS 2F11/2]

Invented by Linda Ariza-McNaughton from Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
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Catalogue Number 151483
Applications ELISA IHC IF IP WB
Antigen/Gene or Protein Targets Zebrafish gut secretory cell epitopes
Reactivity Zebrafish
Relevance Monoclonal antibody which labels all secretory cells within the zebrafish intestinal epithelium.

Background and Research Application
The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. This antibody labels all the gut secretory cells of the zebrafish intestinal epithelium, both mucous and enteroendocrine, corresponding to the class of cells that are lost in MathI mouse mutants. The antibody also labels hepatopancreatic ducts, intrahepatic bile ducts and enteroendocrine.
Anti-2F11 provides an early marker to distinguish cell types and cell polarity in the zebrafish gut.
Host Mouse
Immunogen Lysate of zebrafish intestine
Subclass IgG1
Molecular Weight (kDa) 170, 120, 90 & 35
Myeloma Used Sp2/0-Ag14
Strain Balb/c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
From 2 weeks of development onwards, three categories of 2F11-positive cells could be clearly distinguished in the intestinal epithelium.
This antibody also stains some small basal cells and cells that extend thin processes towards the gut lumen which may be varieties of enteroendocrine cells.

Suggested Dilution for western blot 1:500.

FIS 2F11/2 may also be used to stain stain formalin fixed tissue samples.

Concentration
1mg/ml as standard
Research Area Aquaculture, Cell Cycle, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Developmental Biology, Epigenetics & Nuclear Signalling, Epitope Tags, Stem Cell Biology

References

There are 26 reference entries for this reagent.

View All References

References: 26 entries

Nissim et al. 2019. Nat Genet. 51(9):1308-1314. PMID: 31406347.

View  

Gao et al. 2019. J Mol Cell Biol. 11(6):448-462. PMID: 30428031.

View  

Murdoch et al. 2019. PLoS Pathog. 15(3):e1007381. PMID: 30845179.

View  

Hong et al. 2018. Mol Med Rep. 17(2):2837-2844. PMID: 29257268.

View  

Hong et al. 2018. Mol Med Rep. 17(2):2837-2844. PMID: 29257268.

View  

Freudenblum et al. 2018. Development. 145(3):. PMID: 29386244.

View  

Freudenblum et al. 2018. Development. 145(3):. PMID: 29386244.

View  

Tomé et al. 2015. Sci Rep. 5:14384. PMID: 26399509.

View  

Kimmel et al. 2015. Sci Rep. 5:14241. PMID: 26384018.

IHC

View  

Wang et al. 2015. Dev Dyn. 244(6):724-35. PMID: 25773748.

View  

Ningappa et al. 2015. PLoS One. 10(9):e0138381. PMID: 26379158.

IHC

View  

Flasse et al. 2013. Dev Biol. 376(2):187-97. PMID: 23352790.

IHC

View  

Palha et al. 2013. PLoS Pathog. 9(9):e1003619. PMID: 24039582.

View  

Garnaas et al. 2012. Dev Biol. 372(2):178-89. PMID: 22982668.

IHC

View  

Manfroid et al. 2012. Dev Biol. 366(2):268-78. PMID: 22537488.

View  

Ninov et al. 2012. Development. 139(9):1557-67. PMID: 22492351.

IHC

View  

Poulain et al. 2011. Development. 138(16):3557-68. PMID: 21771809.

IHC

View  

Shin et al. 2011. Development. 138(7):1339-48. PMID: 21385764.

IHC

View  

Matthews et al. 2008. Dev Dyn. 237(1):124-31. PMID: 18095340.

View  

Transcription factor onecut3 regulates intrahepatic biliary development in zebrafish.

Europe PMC ID: 18095340

View  

Dong et al. 2007. Nat Genet. 39(3):397-402. PMID: 17259985.

View  

Fgf10 regulates hepatopancreatic ductal system patterning and differentiation.

Europe PMC ID: 17259985

View  

Bates et al. 2006. Dev Biol. 297(2):374-86. PMID: 16781702.

View  

Distinct signals from the microbiota promote different aspects of zebrafish gut differentiation.

Europe PMC ID: 16781702

View  

Crosnier et al. 2005. Development. 132(5):1093-104. PMID: 15689380.

View  

Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine.

Europe PMC ID: 15689380

View  

Add a reference

References: 26 entries

Nissim et al. 2019. Nat Genet. 51(9):1308-1314. PMID: 31406347.

View  

Gao et al. 2019. J Mol Cell Biol. 11(6):448-462. PMID: 30428031.

View  

Murdoch et al. 2019. PLoS Pathog. 15(3):e1007381. PMID: 30845179.

View  

Hong et al. 2018. Mol Med Rep. 17(2):2837-2844. PMID: 29257268.

View  

Hong et al. 2018. Mol Med Rep. 17(2):2837-2844. PMID: 29257268.

View  

Freudenblum et al. 2018. Development. 145(3):. PMID: 29386244.

View  

Freudenblum et al. 2018. Development. 145(3):. PMID: 29386244.

View  

Tomé et al. 2015. Sci Rep. 5:14384. PMID: 26399509.

View  

Kimmel et al. 2015. Sci Rep. 5:14241. PMID: 26384018.

IHC

View  

Wang et al. 2015. Dev Dyn. 244(6):724-35. PMID: 25773748.

View  

Ningappa et al. 2015. PLoS One. 10(9):e0138381. PMID: 26379158.

IHC

View  

Flasse et al. 2013. Dev Biol. 376(2):187-97. PMID: 23352790.

IHC

View  

Palha et al. 2013. PLoS Pathog. 9(9):e1003619. PMID: 24039582.

View  

Garnaas et al. 2012. Dev Biol. 372(2):178-89. PMID: 22982668.

IHC

View  

Manfroid et al. 2012. Dev Biol. 366(2):268-78. PMID: 22537488.

View  

Ninov et al. 2012. Development. 139(9):1557-67. PMID: 22492351.

IHC

View  

Poulain et al. 2011. Development. 138(16):3557-68. PMID: 21771809.

IHC

View  

Shin et al. 2011. Development. 138(7):1339-48. PMID: 21385764.

IHC

View  

Matthews et al. 2008. Dev Dyn. 237(1):124-31. PMID: 18095340.

View  

Transcription factor onecut3 regulates intrahepatic biliary development in zebrafish.

View  

Dong et al. 2007. Nat Genet. 39(3):397-402. PMID: 17259985.

View  

Fgf10 regulates hepatopancreatic ductal system patterning and differentiation.

View  

Bates et al. 2006. Dev Biol. 297(2):374-86. PMID: 16781702.

View  

Distinct signals from the microbiota promote different aspects of zebrafish gut differentiation.

View  

Crosnier et al. 2005. Development. 132(5):1093-104. PMID: 15689380.

View  

Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine.

View  

Add a reference

Inventor Information