#151483

Anti-Zebrafish gut secretory cell epitopes [FIS 2F11/2]

Cat. #151483

Anti-Zebrafish gut secretory cell epitopes [FIS 2F11/2]

Cat. #: 151483

Sub-type: Primary antibody

Unit size: 100 ug

Availability: 1-2 weeks

Target: Zebrafish gut secretory cell epitopes

Class: Monoclonal

Application: IHC ; ELISA ; IHC ; IF ; IP ; WB

Reactivity: Zebrafish

Host: Mouse

£300.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Linda Ariza-McNaughton

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Target Details
Applications
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: Anti-Zebrafish gut secretory cell epitopes [FIS 2F11/2]
  • Clone: FIS 2F11/2
  • Tool sub type: Primary antibody
  • Class: Monoclonal
  • Conjugation: Unconjugated
  • Molecular weight: 170 kDa, 120 kDa, 90 kDa and 35 kDa
  • Strain: Balb/c
  • Reactivity: Zebrafish
  • Host: Mouse
  • Application: IHC ; ELISA ; IHC ; IF ; IP ; WB
  • Description: Monoclonal antibody which labels all secretory cells within the zebrafish intestinal epithelium.
  • Immunogen: Lysate of zebrafish intestine
  • Isotype: IgG1
  • Myeloma used: Sp2/0-Ag14

Target Details

  • Target: Zebrafish gut secretory cell epitopes
  • Molecular weight: 170 kDa, 120 kDa, 90 kDa and 35 kDa
  • Target background: The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. This antibody labels all the gut secretory cells of the zebrafish intestinal epithelium, both mucous and enteroendocrine, corresponding to the class of cells that are lost in MathI mouse mutants. The antibody also labels hepatopancreatic ducts, intrahepatic bile ducts and enteroendocrine. Anti-2F11 provides an early marker to distinguish cell types and cell polarity in the zebrafish gut.

Applications

  • Application: IHC ; ELISA ; IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Unit size: 100 ug
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4° C

Related Tools

  • Related tools: Anti-Zebrafish Basolateral Pole of Cells [FIS 2H9/1]

References

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  • Murdoch et al. 2019. PLoS Pathog. 15(3):e1007381. PMID: 30845179.
  • Hong et al. 2018. Mol Med Rep. 17(2):2837-2844. PMID: 29257268.
  • Hong et al. 2018. Mol Med Rep. 17(2):2837-2844. PMID: 29257268.
  • Freudenblum et al. 2018. Development. 145(3):. PMID: 29386244.
  • Freudenblum et al. 2018. Development. 145(3):. PMID: 29386244.
  • Tom et al. 2015. Sci Rep. 5:14384. PMID: 26399509.
  • Kimmel et al. 2015. Sci Rep. 5:14241. PMID: 26384018.
  • Wang et al. 2015. Dev Dyn. 244(6):724-35. PMID: 25773748.
  • Ningappa et al. 2015. PLoS One. 10(9):e0138381. PMID: 26379158.
  • Flasse et al. 2013. Dev Biol. 376(2):187-97. PMID: 23352790.
  • Palha et al. 2013. PLoS Pathog. 9(9):e1003619. PMID: 24039582.
  • Garnaas et al. 2012. Dev Biol. 372(2):178-89. PMID: 22982668.
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  • Matthews et al. 2008. Dev Dyn. 237(1):124-31. PMID: 18095340.
  • Transcription factor onecut3 regulates intrahepatic biliary development in zebrafish.
  • Dong et al. 2007. Nat Genet. 39(3):397-402. PMID: 17259985.
  • Fgf10 regulates hepatopancreatic ductal system patterning and differentiation.
  • Bates et al. 2006. Dev Biol. 297(2):374-86. PMID: 16781702.
  • Distinct signals from the microbiota promote different aspects of zebrafish gut differentiation.
  • Crosnier et al. 2005. Development. 132(5):1093-104. PMID: 15689380.
  • Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine.