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|Antigen/Gene or Protein Targets||p300|
|Parental Line||HCT 116|
|Relevance||The HCT 116 p300 KO Cell Line is a tool for the in vitro study of p300 in colon carcinoma, and specifically of its role in p53-dependent apoptosis, cellular adhesion and migration. Cell lines available are p300 WT (HCT116), p300 KO Clone D10 (HCT116), p300 KO Clone F5 (HCT116), p300 KO Clone F2 (HCT116). Experiments conducted should be using at least 2 of the 3 KO cell lines. The best 2 KO clones are D10 and F5. Anyone requesting the cell line should use the parental cell line that the KOs were generated from. The p300 KO lines demonstrate an ‘aggressive’ cancer phenotype in vitro, with loss of cell-cell adhesion, defects in cell-matrix adhesion, and increased migration through collagen matrices. p300 is a transcriptional cofactor involved in regulating multiple cellular processes including cell cycle regulation, proliferation, differentiation, apoptosis, DNA damage repair and adhesion properties. Somatic inactivating mutations of p300 are associated with several cancers including breast, colorectal and gastric cancers.|
|Research Area||Cancer, Cell Cycle, Cell Structure and Motility, Drug Discovery & Development|
|Growth/Phenotype Keywords||Adhesion properties; invasion; migration|
|Recommended Growing Conditions||McCoy's 5A medium + 10% FCS|
|Notes||CBP was not manipulated in this cell line|
References: 5 entries
Wallace et al. 2015. PLoS Pathog. 11(3):e1004687. PMID: 25803638.
Iyer et al. 2007. Oncogene. 26(1):21-9. PMID: 16878158.
Bundy et al. 2006. Cancer Res. 66(15):7606-14. PMID: 16885360.
Krubasik et al. 2006. Br J Cancer. 94(9):1326-32. PMID: 16622451.
Iyer et al. 2004. Proc Natl Acad Sci U S A. 101(19):7386-91. PMID: 15123817.
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