#151479

HCT 116 p300 KO [D10] Cell Line

Cat. #151479

HCT 116 p300 KO [D10] Cell Line

Cat. #: 151479

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 10-12 weeks

Organism: Human

Tissue: Colon

Disease: Cancer

Model: Knock-Out

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Carlos Caldas

Institute: Cancer Research UK Cambridge Institute

Tool Details
Target Details
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: HCT 116 p300 KO [D10] Cell Line
  • Tool sub type: Continuous
  • Parental cell: HCT 116
  • Organism: Human
  • Tissue: Colon
  • Disease: Cancer
  • Growth properties: Adhesion properties; invasion; migration
  • Model: Knock-Out
  • Description: The HCT 116 p300 KO Cell Line is a tool for the in vitro study of p300 in colon carcinoma, and specifically of its role in p53-dependent apoptosis, cellular adhesion and migration. Cell lines available are p300 WT (HCT116), p300 KO Clone D10 (HCT116), p300 KO Clone F5 (HCT116), p300 KO Clone F2 (HCT116). Experiments conducted should be using at least 2 of the 3 KO cell lines. The best 2 KO clones are D10 and F5. Anyone requesting the cell line should use the parental cell line that the KOs ...
  • Biosafety level: 1
  • Additional notes: CBP was not manipulated in this cell line
  • Recommended controls: HCT 116 parental line
  • Cellosaurus id: CVCL_HG27

Target Details

  • Target: p300

Handling

  • Format: Frozen
  • Growth medium: McCoy's 5A medium + 10% FCS
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: -20° C
  • Mycoplasma free: Yes

Related Tools

  • Related tools: HCT 116 p300 WT Cell Line ; HCT 116 p300 KO [F5] Cell Line ; HCT 116 p300 KO [F2] Cell Line ; HCT 116 p300 KO Rescue Cell Line

References

  • Wallace et al. 2015. PLoS Pathog. 11(3):e1004687. PMID: 25803638.
  • ?-HPV 5 and 8 E6 disrupt homology dependent double strand break repair by attenuating BRCA1 and BRCA2 expression and foci formation.
  • Iyer et al. 2007. Oncogene. 26(1):21-9. PMID: 16878158.
  • Bundy et al. 2006. Cancer Res. 66(15):7606-14. PMID: 16885360.
  • Metabolic consequences of p300 gene deletion in human colon cancer cells.
  • p300 is required for orderly G1/S transition in human cancer cells.
  • Krubasik et al. 2006. Br ...