MEF Raf1 FF/FF KI Cell Line
Invented at University Of Leicester
Raf-1FF/FF knockin mouse
Invented at University Of Leicester
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 151477 |
| Antigen/Gene or Protein Targets | Raf1 Y340F Y341F (inactive mutant) |
| Disease Keywords | Cancer; Apoptosis |
| Relevance | A knockin of inactive Raf-1FF/FF mutant; can be use for in vivo study of inactive Raf-1 mutant, and Ras signalling; |
| Production Details | A Raf-1 targeting vector, encoding Raf-1 with a substitution at residues 340 (Y to F) and 341 (Y to F), and a loxP flanked resistance cassette, was transfected into 129Ola ES cells. Properly targeted ES cells containing a homologous recombination event were selected, and transfected with a Cre expressing vector to excise the resistance cassette. Targeted ES cells were then injected into C57BL/6 blastocysts. Chimeric offspring were bred with C57BL/6 mice to yield mice heterozygous for the mutant allele. |
| Research Area | Cancer, Cell Signaling & Signal Transduction, Epigenetics & Nuclear Signalling, Genetic Studies Tools, Neurobiology |
| Notes | Genetic Background / Cross History : C57BL/6 |
References: 2 entries
Hüser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227.
MEK kinase activity is not necessary for Raf-1 function.
Europe PMC ID: 11296227
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References: 2 entries
Hüser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227.
MEK kinase activity is not necessary for Raf-1 function.
Add a reference
