Cat. #151477
Raf-1FF/FF knockin mouse
Cat. #: 151477
Sub-type: Mouse
Availability: 6-8 weeks
Disease: Cancer; Apoptosis
Model: Knock-In
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Contributor
Inventor: Catrin Pritchard
Institute: University of Leicester
Tool Details
*FOR RESEARCH USE ONLY
- Tool name: Raf-1FF/FF knockin mouse
- Tool sub type: Mouse
- Disease: Cancer; Apoptosis
- Model: Knock-In
- Description: A knockin of inactive Raf-1FF/FF mutant; can be use for in vivo study of inactive Raf-1 mutant, and Ras signalling;
- Genetic background: A Raf-1 targeting vector, encoding Raf-1 with a substitution at residues 340 (Y to F) and 341 (Y to F), and a loxP flanked resistance cassette, was transfected into 129Ola ES cells. Properly targeted ES cells containing a homologous recombination event were selected, and transfected with a Cre expressing vector to excise the resistance cassette. Targeted ES cells were then injected into C57BL/6 blastocysts. Chimeric offspring were bred with C57BL/6 mice to yield mice heterozygous for the mutant allele.
- Strain: C57BL/6
- Production details: A Raf-1 targeting vector, encoding Raf-1 with a substitution at residues 340 (Y to F) and 341 (Y to F), and a loxP flanked resistance cassette, was transfected into 129Ola ES cells. Properly targeted ES cells containing a homologous recombination event were selected, and transfected with a Cre expressing vector to excise the resistance cassette. Targeted ES cells were then injected into C57BL/6 blastocysts. Chimeric offspring were bred with C57BL/6 mice to yield mice heterozygous for the mutant allele.
- Additional notes: Genetic Background / Cross History : C57BL/6
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: Raf1 Y340F Y341F (inactive mutant)
Related Tools
- Related tools: MEF Raf1 FF/FF KI Cell Line
References
- H
ser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227. - MEK kinase activity is not necessary for Raf-1 function.
