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Raf-1FF/FF knockin mouse


Catalogue Number 151477
Antigen/Gene or Protein Targets Raf1 Y340F Y341F (inactive mutant)
Disease Keywords Cancer; Apoptosis
Relevance A knockin of inactive Raf-1FF/FF mutant; can be use for in vivo study of inactive Raf-1 mutant, and Ras signalling;
Production Details A Raf-1 targeting vector, encoding Raf-1 with a substitution at residues 340 (Y to F) and 341 (Y to F), and a loxP flanked resistance cassette, was transfected into 129Ola ES cells. Properly targeted ES cells containing a homologous recombination event were selected, and transfected with a Cre expressing vector to excise the resistance cassette. Targeted ES cells were then injected into C57BL/6 blastocysts. Chimeric offspring were bred with C57BL/6 mice to yield mice heterozygous for the mutant allele.
Research Area Cancer, Cell Signaling & Signal Transduction, Epigenetics & Nuclear Signalling, Genetic Studies Tools, Neurobiology
Notes Genetic Background / Cross History : C57BL/6

References: 1 entry

Hüser et al. 2001. EMBO J. 20(8):1940-51. PMID: 11296227.

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