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Anti-FCER2 [BU38]


Catalogue Number 151430
Applications FACS IHC IF IP WB
Antigen/Gene or Protein Targets Fc epsilon RII (FCER2, CD23)
Synonyms Fc Fragment Of IgE Receptor II; Immunoglobulin E-Binding Factor; Lymphocyte IgE Receptor; Fc Epsilon Receptor II; CD23 Antigen; BLAST-2; CLEC4J; IGEBF; FCE2; C-Type Lectin Domain Family 4; Member; Immunoglobulin Epsilon-Chain; CD23
Reactivity Human
Relevance Fc epsilon RII (CD23), a low-affinity IgE receptor, is widely expressed on the surface of a number of cell types. Fc epsilon RII mediates numerous IgE-related immune responses. Both membrane and soluble Fc epsilon RII play important roles in allergic responses.
Host Mouse
Immunogen Full length native CD23 of human origin
Subclass IgG1
Positive Control Follicular lymphoma or tonsil (mantle cell lymphomas are negative)
Myeloma Used NS0
Recommended Growing Conditions RPMI 1640 + 10% FCS
Notes Flow Cytometry: 5x10e5 ficoll prepared human peripheral blood mononuclear cells were washed and preincubated 5 min with 20ul of 250ug/ml human IgG (To block non specific binding) after which they were1.2 washed and incubated 45 min on ice with 80ul of anti-0.2 0. Binding of anti-CD23 antibody to human cell lines 0 10 20 30ug/ml PBL Nalm-6 1 0.8 0.6 CD23 antibody at a concentration of 10ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A net 5% sub population of the cells stained positive with a mean shift of 0.94 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
Research Area Immunology, Stem Cell Biology

References: 17 entries

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