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Anti-FCER2 [BU38]

Info

Catalogue Number 151430
Applications FACS IHC IF IP WB
Antigen/Gene or Protein Targets Fc epsilon RII (FCER2, CD23)
Synonyms Fc Fragment Of IgE Receptor II; Immunoglobulin E-Binding Factor; Lymphocyte IgE Receptor; Fc Epsilon Receptor II; CD23 Antigen; BLAST-2; CLEC4J; IGEBF; FCE2; C-Type Lectin Domain Family 4; Member; Immunoglobulin Epsilon-Chain; CD23
Reactivity Human
Relevance Fc epsilon RII (CD23), a low-affinity IgE receptor, is widely expressed on the surface of a number of cell types. Fc epsilon RII mediates numerous IgE-related immune responses. Both membrane and soluble Fc epsilon RII play important roles in allergic responses.
Host Mouse
Immunogen Full length native CD23 of human origin
Subclass IgG1
Positive Control Follicular lymphoma or tonsil (mantle cell lymphomas are negative)
Myeloma Used NS0
Recommended Growing Conditions RPMI 1640 + 10% FCS
Notes Flow Cytometry: 5x10e5 ficoll prepared human peripheral blood mononuclear cells were washed and preincubated 5 min with 20ul of 250ug/ml human IgG (To block non specific binding) after which they were1.2 washed and incubated 45 min on ice with 80ul of anti-0.2 0. Binding of anti-CD23 antibody to human cell lines 0 10 20 30ug/ml PBL Nalm-6 1 0.8 0.6 CD23 antibody at a concentration of 10ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A net 5% sub population of the cells stained positive with a mean shift of 0.94 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
Research Area Immunology, Stem Cell Biology

References: 30 entries

Aubry JP. et al. 1987. Leucocyte Typing III. Oxford University Press-Oxford, New York, Tokyo

Knapp W. et al. 1989. Leukocyte Typing IV. Oxford University Press

Leucocyte Typing VI, Garland Publishing (1998)

Schlossman S.F. et al. 1995. Leukocyte Typing V. Oxford University Press

Maeda et al. 2002. J Histochem Cytochem. 50(11):1475-86. PMID: 12417613.

Immunohistochemical recognition of human follicular dendritic cells (FDCs) in routinely processed paraffin sections.

Europe PMC ID: 12417613

Karagiannis et al. 2001. Immunology. 103(3):319-31. PMID: 11454061.

Endocytosis and recycling of the complex between CD23 and HLA-DR in human B cells.

Europe PMC ID: 11454061

Sancho et al. 2000. J Immunol. 165(7):3868-75. PMID: 11034393.

Functional analysis of ligand-binding and signal transduction domains of CD69 and CD23 C-type lectin leukocyte receptors.

Europe PMC ID: 11034393

Chen et al. 2000. Appl Immunohistochem Mol Morphol. 8(1):1-11. PMID: 10937042.

Classification of small B-cell lymphoid neoplasms using a paraffin section immunohistochemical panel.

Europe PMC ID: 10937042

Sano et al. 1999. Proc Assoc Am Physicians. 111(1):82-91. PMID: 9893160.

Upregulated surface expression of intracellularly sequestered Igepsilon receptors (FcepsilonRII/CD23) following activation in human peripheral blood eosinophils.

Europe PMC ID: 9893160

Kroft et al. 1998. Mod Pathol. 11(10):967-70. PMID: 9796724.

Evaluation of CD23 expression in paraffin-embedded gastric lymphomas of mucosa-associated lymphoid tissue.

Europe PMC ID: 9796724

Sarsfield et al. 1996. J Pathol. 180(1):18-25. PMID: 8943810.

Kumar et al. 1996. Mod Pathol. 9(9):925-9. PMID: 8878025.

Use of CD23 (BU38) on paraffin sections in the diagnosis of small lymphocytic lymphoma and mantle cell lymphoma.

Europe PMC ID: 8878025

A study of accessory cells in the acquired lymphoid tissue of helicobacter gastritis.

Europe PMC ID: 8943810

Bonnefoy et al. 1995. Curr Opin Immunol. 7(3):355-9. PMID: 7546400.

CD23 and B-cell activation.

Europe PMC ID: 7546400

Hardie et al. 1993. Eur J Immunol. 23(5):997-1004. PMID: 8477815.

Quantitative analysis of molecules which distinguish functional compartments within germinal centers.

Europe PMC ID: 8477815

Murray et al. 1991. J Pathol. 165(2):125-8. PMID: 1744798.

CD23 expression in non-Hodgkin lymphoma: immunohistochemical demonstration using the antibody BU38 on paraffin sections.

Europe PMC ID: 1744798

Hellen et al. 1991. J Clin Pathol. 44(4):293-6. PMID: 1827636.

Immunohistochemical demonstration of CD23 expression on lymphocytes in rheumatoid synovitis.

Europe PMC ID: 1827636

Rowlands et al. 1990. J Pathol. 160(3):239-43. PMID: 2139890.

Immunohistochemical determination of CD23 expression in Hodgkin's disease using paraffin sections.

Europe PMC ID: 2139890


Add a reference

References: 30 entries

Aubry JP. et al. 1987. Leucocyte Typing III. Oxford University Press-Oxford, New York, Tokyo

Knapp W. et al. 1989. Leukocyte Typing IV. Oxford University Press

Leucocyte Typing VI, Garland Publishing (1998)

Schlossman S.F. et al. 1995. Leukocyte Typing V. Oxford University Press

Maeda et al. 2002. J Histochem Cytochem. 50(11):1475-86. PMID: 12417613.

Immunohistochemical recognition of human follicular dendritic cells (FDCs) in routinely processed paraffin sections.

Europe PMC ID: 12417613

Karagiannis et al. 2001. Immunology. 103(3):319-31. PMID: 11454061.

Endocytosis and recycling of the complex between CD23 and HLA-DR in human B cells.

Europe PMC ID: 11454061

Sancho et al. 2000. J Immunol. 165(7):3868-75. PMID: 11034393.

Functional analysis of ligand-binding and signal transduction domains of CD69 and CD23 C-type lectin leukocyte receptors.

Europe PMC ID: 11034393

Chen et al. 2000. Appl Immunohistochem Mol Morphol. 8(1):1-11. PMID: 10937042.

Classification of small B-cell lymphoid neoplasms using a paraffin section immunohistochemical panel.

Europe PMC ID: 10937042

Sano et al. 1999. Proc Assoc Am Physicians. 111(1):82-91. PMID: 9893160.

Upregulated surface expression of intracellularly sequestered Igepsilon receptors (FcepsilonRII/CD23) following activation in human peripheral blood eosinophils.

Europe PMC ID: 9893160

Kroft et al. 1998. Mod Pathol. 11(10):967-70. PMID: 9796724.

Evaluation of CD23 expression in paraffin-embedded gastric lymphomas of mucosa-associated lymphoid tissue.

Europe PMC ID: 9796724

Sarsfield et al. 1996. J Pathol. 180(1):18-25. PMID: 8943810.

Kumar et al. 1996. Mod Pathol. 9(9):925-9. PMID: 8878025.

Use of CD23 (BU38) on paraffin sections in the diagnosis of small lymphocytic lymphoma and mantle cell lymphoma.

Europe PMC ID: 8878025

A study of accessory cells in the acquired lymphoid tissue of helicobacter gastritis.

Europe PMC ID: 8943810

Bonnefoy et al. 1995. Curr Opin Immunol. 7(3):355-9. PMID: 7546400.

CD23 and B-cell activation.

Europe PMC ID: 7546400

Hardie et al. 1993. Eur J Immunol. 23(5):997-1004. PMID: 8477815.

Quantitative analysis of molecules which distinguish functional compartments within germinal centers.

Europe PMC ID: 8477815

Murray et al. 1991. J Pathol. 165(2):125-8. PMID: 1744798.

CD23 expression in non-Hodgkin lymphoma: immunohistochemical demonstration using the antibody BU38 on paraffin sections.

Europe PMC ID: 1744798

Hellen et al. 1991. J Clin Pathol. 44(4):293-6. PMID: 1827636.

Immunohistochemical demonstration of CD23 expression on lymphocytes in rheumatoid synovitis.

Europe PMC ID: 1827636

Rowlands et al. 1990. J Pathol. 160(3):239-43. PMID: 2139890.

Immunohistochemical determination of CD23 expression in Hodgkin's disease using paraffin sections.

Europe PMC ID: 2139890


Add a reference

References: 30 entries

Aubry JP. et al. 1987. Leucocyte Typing III. Oxford University Press-Oxford, New York, Tokyo

Knapp W. et al. 1989. Leukocyte Typing IV. Oxford University Press

Leucocyte Typing VI, Garland Publishing (1998)

Schlossman S.F. et al. 1995. Leukocyte Typing V. Oxford University Press

Maeda et al. 2002. J Histochem Cytochem. 50(11):1475-86. PMID: 12417613.

Immunohistochemical recognition of human follicular dendritic cells (FDCs) in routinely processed paraffin sections.

Karagiannis et al. 2001. Immunology. 103(3):319-31. PMID: 11454061.

Endocytosis and recycling of the complex between CD23 and HLA-DR in human B cells.

Sancho et al. 2000. J Immunol. 165(7):3868-75. PMID: 11034393.

Functional analysis of ligand-binding and signal transduction domains of CD69 and CD23 C-type lectin leukocyte receptors.

Chen et al. 2000. Appl Immunohistochem Mol Morphol. 8(1):1-11. PMID: 10937042.

Classification of small B-cell lymphoid neoplasms using a paraffin section immunohistochemical panel.

Sano et al. 1999. Proc Assoc Am Physicians. 111(1):82-91. PMID: 9893160.

Upregulated surface expression of intracellularly sequestered Igepsilon receptors (FcepsilonRII/CD23) following activation in human peripheral blood eosinophils.

Kroft et al. 1998. Mod Pathol. 11(10):967-70. PMID: 9796724.

Evaluation of CD23 expression in paraffin-embedded gastric lymphomas of mucosa-associated lymphoid tissue.

Sarsfield et al. 1996. J Pathol. 180(1):18-25. PMID: 8943810.

Kumar et al. 1996. Mod Pathol. 9(9):925-9. PMID: 8878025.

Use of CD23 (BU38) on paraffin sections in the diagnosis of small lymphocytic lymphoma and mantle cell lymphoma.

A study of accessory cells in the acquired lymphoid tissue of helicobacter gastritis.

Bonnefoy et al. 1995. Curr Opin Immunol. 7(3):355-9. PMID: 7546400.

CD23 and B-cell activation.

Hardie et al. 1993. Eur J Immunol. 23(5):997-1004. PMID: 8477815.

Quantitative analysis of molecules which distinguish functional compartments within germinal centers.

Murray et al. 1991. J Pathol. 165(2):125-8. PMID: 1744798.

CD23 expression in non-Hodgkin lymphoma: immunohistochemical demonstration using the antibody BU38 on paraffin sections.

Hellen et al. 1991. J Clin Pathol. 44(4):293-6. PMID: 1827636.

Immunohistochemical demonstration of CD23 expression on lymphocytes in rheumatoid synovitis.

Rowlands et al. 1990. J Pathol. 160(3):239-43. PMID: 2139890.

Immunohistochemical determination of CD23 expression in Hodgkin's disease using paraffin sections.


Add a reference


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