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Anti-BrdU [BU20a]
RECENTLY UPDATED

Invented by Dr Jacqueline Cordell at University of Oxford

Info

Catalogue Number 151340
Applications FACS IHC IF
Antigen/Gene or Protein Targets Bromodeoxyuridine (BrdU)
Reactivity Human
Relevance
RECENTLY UPDATED
Monoclonal antibody detects proliferating cells in the S phase via binding the synthetic nucleoside BrdU.

Background and Research Application
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. It can be incorporated into the newly synthesized DNA of replicating cells, within the S phase of the cell cycle instead of thymidine.

Anti-BrdU works well in immunohistochemistry to identify cells in tissue sections or cytospin preparations that have incorporated BrdU. This indicates cells that were actively replicating their DNA. Anti-BrdU stains BrdU incorporated into the nuclei of a wide range of cell types, including human tonsil lymphoid cells and human tumours growing in nude mice. It is also useful to identify cells in S phase during FACS analysis. Anti-BrdU can be used to analyse the phenotype of S-phase cells and in co-localizing antigen expression and BrdU incorporation in tissue sections.

This antibody was created to replace similar use polyclonal antibodies which frequently cross reacted with other nucleotides. Binding of this antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
Host Mouse
Immunogen Bromodeoxyuridine conjugated to BSA
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM)
Notes
RECENTLY UPDATED
Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Ultroser G can be used at 1% if the cells are not growing well.
This monoclonal antibody can be used in both human and animal cells.
A double-labelling technique can be used with this antibody, where cells or tissue sections are first labelled with an immunoperoxidase procedure for a cellular antigen and then BrdU is then detected by indirect immunofluorescence.

Concentration
1mg/ml as standard
Research Area Cell Cycle, Cell Type or Organelle Marker, Epigenetics & Nuclear Signalling, Neurobiology

References

There are 28 reference entries for this reagent.

View All References

References: 28 entries
RECENTLY UPDATED

Desamero et al. 2019. Sci Rep. 9(1):19635. PMID: 31873082.

Helbling-Leclerc et al. 2019. Sci Rep. 9(1):17024. PMID: 31745226.

Paul et al. 2019. J Immunother Cancer. 7(1):208. PMID: 31387637.

Dick et al. 2019. Nat Immunol. 20(5):664. PMID: 30862954.

Xu et al. 2019. Sci Adv. 5(4):eaav4570. PMID: 31032410.

Jimenez et al. 2019. Front Immunol. 10:2183. PMID: 31620123.

Zilio et al. 2017. J Immunol. :. PMID: 28396317.

4PD Functionalized Dendrimers: A Flexible Tool for In Vivo Gene Silencing of Tumor-Educated Myeloid Cells.

Europe PMC ID: 28396317

Maternal obesity leads to increased proliferation and numbers of astrocytes in the developing fetal and neonatal mouse hypothalamus.

Europe PMC ID: 27326907

Kim et al. 2016. Int J Dev Neurosci. :. PMID: 27326907.

Yao et al. 2016. Sci Rep. 6:26050. PMID: 27198662.

IF

Functional assessment of glioma pathogenesis by in vivo multi-parametric magnetic resonance imaging and in vitro analyses.

Europe PMC ID: 27198662

Zhao et al. 2015. Oncogene. :. PMID: 26657156.

FACS

CBP/catenin antagonist safely eliminates drug-resistant leukemia-initiating cells.

Europe PMC ID: 26657156

Intratracheal cell transfer demonstrates the profibrotic potential of resident fibroblasts in pulmonary fibrosis.

Europe PMC ID: 26456579

Tsukui et al. 2015. Am J Pathol. :. PMID: 26456579.

Cui et al. 2015. Cell. 161(4):750-61. PMID: 25957683.

FACS

IL-7-Induced Glycerol Transport and TAG Synthesis Promotes Memory CD8+ T Cell Longevity.

Europe PMC ID: 25957683

Sheikh et al. 2015. Oncogene. PMID: 25772242.

FACS

MOZ (MYST3, KAT6A) inhibits senescence via the INK4A-ARF pathway.

Europe PMC ID: 25772242

Lin et al. 2013. Invest Ophthalmol Vis Sci. 54(3):1920-30. PMID: 23439595.

Involvement of SDF1a and STAT3 in granulocyte colony-stimulating factor rescues optic ischemia-induced retinal function loss by mobilizing hematopoietic stem cells.

Europe PMC ID: 23439595

Innis et al. 2010. Am J Physiol Gastrointest Liver Physiol. 299(6):G1376-85. PMID: 20864654.

Perinatal lipid nutrition alters early intestinal development and programs the response to experimental colitis in young adult rats.

Europe PMC ID: 20864654

Zindy et al. 2006. Proc Natl Acad Sci U S A. 103(31):11579-83. PMID: 16864777.

N-Myc and the cyclin-dependent kinase inhibitors p18Ink4c and p27Kip1 coordinately regulate cerebellar development.

Europe PMC ID: 16864777

Magaud et al. 1989. J Histochem Cytochem. 37(10):1517-27. PMID: 2476478.

Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine.

Europe PMC ID: 2476478


Add a reference

References: 28 entries
RECENTLY UPDATED

Desamero et al. 2019. Sci Rep. 9(1):19635. PMID: 31873082.

Helbling-Leclerc et al. 2019. Sci Rep. 9(1):17024. PMID: 31745226.

Paul et al. 2019. J Immunother Cancer. 7(1):208. PMID: 31387637.

Dick et al. 2019. Nat Immunol. 20(5):664. PMID: 30862954.

Xu et al. 2019. Sci Adv. 5(4):eaav4570. PMID: 31032410.

Jimenez et al. 2019. Front Immunol. 10:2183. PMID: 31620123.

Zilio et al. 2017. J Immunol. :. PMID: 28396317.

4PD Functionalized Dendrimers: A Flexible Tool for In Vivo Gene Silencing of Tumor-Educated Myeloid Cells.

Maternal obesity leads to increased proliferation and numbers of astrocytes in the developing fetal and neonatal mouse hypothalamus.

Kim et al. 2016. Int J Dev Neurosci. :. PMID: 27326907.

Yao et al. 2016. Sci Rep. 6:26050. PMID: 27198662.

IF

Functional assessment of glioma pathogenesis by in vivo multi-parametric magnetic resonance imaging and in vitro analyses.

Zhao et al. 2015. Oncogene. :. PMID: 26657156.

FACS

CBP/catenin antagonist safely eliminates drug-resistant leukemia-initiating cells.

Intratracheal cell transfer demonstrates the profibrotic potential of resident fibroblasts in pulmonary fibrosis.

Tsukui et al. 2015. Am J Pathol. :. PMID: 26456579.

Cui et al. 2015. Cell. 161(4):750-61. PMID: 25957683.

FACS

IL-7-Induced Glycerol Transport and TAG Synthesis Promotes Memory CD8+ T Cell Longevity.

Sheikh et al. 2015. Oncogene. PMID: 25772242.

FACS

MOZ (MYST3, KAT6A) inhibits senescence via the INK4A-ARF pathway.

Lin et al. 2013. Invest Ophthalmol Vis Sci. 54(3):1920-30. PMID: 23439595.

Involvement of SDF1a and STAT3 in granulocyte colony-stimulating factor rescues optic ischemia-induced retinal function loss by mobilizing hematopoietic stem cells.

Innis et al. 2010. Am J Physiol Gastrointest Liver Physiol. 299(6):G1376-85. PMID: 20864654.

Perinatal lipid nutrition alters early intestinal development and programs the response to experimental colitis in young adult rats.

Zindy et al. 2006. Proc Natl Acad Sci U S A. 103(31):11579-83. PMID: 16864777.

N-Myc and the cyclin-dependent kinase inhibitors p18Ink4c and p27Kip1 coordinately regulate cerebellar development.

Magaud et al. 1989. J Histochem Cytochem. 37(10):1517-27. PMID: 2476478.

Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine.


Add a reference