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Anti-BrdU [BU20a]

Invented by Dr Jacqueline Cordell at University of Oxford

Info

Catalogue Number 151340
Applications FACS IHC IF
Antigen/Gene or Protein Targets Bromodeoxyuridine (BrdU)
Reactivity Human
Relevance BrdU is an analogue of thymidine and is taken up into the DNA of proliferating cells. Bu20a works well in immunohistochemistry to identify cells in tissue sections or cytospin preparations that have incorporated BrdU. It is also useful to identify cells in S phase during FACS analysis.
Host Mouse
Immunogen Bromodeoxyuridine conjugated to BSA
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM)
Notes Ultroser G can be used at 1% if the cells are not growing well.
Research Area Cell Cycle, Cell Type or Organelle Marker, Epigenetics & Nuclear Signalling, Neurobiology

References: 22 entries

Zilio et al. 2017. J Immunol. :. PMID: 28396317.

4PD Functionalized Dendrimers: A Flexible Tool for In Vivo Gene Silencing of Tumor-Educated Myeloid Cells.

Europe PMC ID: 28396317

Maternal obesity leads to increased proliferation and numbers of astrocytes in the developing fetal and neonatal mouse hypothalamus.

Europe PMC ID: 27326907

Kim et al. 2016. Int J Dev Neurosci. :. PMID: 27326907.

Yao et al. 2016. Sci Rep. 6:26050. PMID: 27198662.

IF

Functional assessment of glioma pathogenesis by in vivo multi-parametric magnetic resonance imaging and in vitro analyses.

Europe PMC ID: 27198662

Zhao et al. 2015. Oncogene. :. PMID: 26657156.

FACS

CBP/catenin antagonist safely eliminates drug-resistant leukemia-initiating cells.

Europe PMC ID: 26657156

Intratracheal cell transfer demonstrates the profibrotic potential of resident fibroblasts in pulmonary fibrosis.

Europe PMC ID: 26456579

Tsukui et al. 2015. Am J Pathol. :. PMID: 26456579.

Cui et al. 2015. Cell. 161(4):750-61. PMID: 25957683.

FACS

IL-7-Induced Glycerol Transport and TAG Synthesis Promotes Memory CD8+ T Cell Longevity.

Europe PMC ID: 25957683

Sheikh et al. 2015. Oncogene. PMID: 25772242.

FACS

MOZ (MYST3, KAT6A) inhibits senescence via the INK4A-ARF pathway.

Europe PMC ID: 25772242

Lin et al. 2013. Invest Ophthalmol Vis Sci. 54(3):1920-30. PMID: 23439595.

Involvement of SDF1a and STAT3 in granulocyte colony-stimulating factor rescues optic ischemia-induced retinal function loss by mobilizing hematopoietic stem cells.

Europe PMC ID: 23439595

Innis et al. 2010. Am J Physiol Gastrointest Liver Physiol. 299(6):G1376-85. PMID: 20864654.

Perinatal lipid nutrition alters early intestinal development and programs the response to experimental colitis in young adult rats.

Europe PMC ID: 20864654

Zindy et al. 2006. Proc Natl Acad Sci U S A. 103(31):11579-83. PMID: 16864777.

N-Myc and the cyclin-dependent kinase inhibitors p18Ink4c and p27Kip1 coordinately regulate cerebellar development.

Europe PMC ID: 16864777

Magaud et al. 1989. J Histochem Cytochem. 37(10):1517-27. PMID: 2476478.

Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine.

Europe PMC ID: 2476478


Add a reference

References: 22 entries

Zilio et al. 2017. J Immunol. :. PMID: 28396317.

4PD Functionalized Dendrimers: A Flexible Tool for In Vivo Gene Silencing of Tumor-Educated Myeloid Cells.

Europe PMC ID: 28396317

Maternal obesity leads to increased proliferation and numbers of astrocytes in the developing fetal and neonatal mouse hypothalamus.

Europe PMC ID: 27326907

Kim et al. 2016. Int J Dev Neurosci. :. PMID: 27326907.

Yao et al. 2016. Sci Rep. 6:26050. PMID: 27198662.

IF

Functional assessment of glioma pathogenesis by in vivo multi-parametric magnetic resonance imaging and in vitro analyses.

Europe PMC ID: 27198662

Zhao et al. 2015. Oncogene. :. PMID: 26657156.

FACS

CBP/catenin antagonist safely eliminates drug-resistant leukemia-initiating cells.

Europe PMC ID: 26657156

Intratracheal cell transfer demonstrates the profibrotic potential of resident fibroblasts in pulmonary fibrosis.

Europe PMC ID: 26456579

Tsukui et al. 2015. Am J Pathol. :. PMID: 26456579.

Cui et al. 2015. Cell. 161(4):750-61. PMID: 25957683.

FACS

IL-7-Induced Glycerol Transport and TAG Synthesis Promotes Memory CD8+ T Cell Longevity.

Europe PMC ID: 25957683

Sheikh et al. 2015. Oncogene. PMID: 25772242.

FACS

MOZ (MYST3, KAT6A) inhibits senescence via the INK4A-ARF pathway.

Europe PMC ID: 25772242

Lin et al. 2013. Invest Ophthalmol Vis Sci. 54(3):1920-30. PMID: 23439595.

Involvement of SDF1a and STAT3 in granulocyte colony-stimulating factor rescues optic ischemia-induced retinal function loss by mobilizing hematopoietic stem cells.

Europe PMC ID: 23439595

Innis et al. 2010. Am J Physiol Gastrointest Liver Physiol. 299(6):G1376-85. PMID: 20864654.

Perinatal lipid nutrition alters early intestinal development and programs the response to experimental colitis in young adult rats.

Europe PMC ID: 20864654

Zindy et al. 2006. Proc Natl Acad Sci U S A. 103(31):11579-83. PMID: 16864777.

N-Myc and the cyclin-dependent kinase inhibitors p18Ink4c and p27Kip1 coordinately regulate cerebellar development.

Europe PMC ID: 16864777

Magaud et al. 1989. J Histochem Cytochem. 37(10):1517-27. PMID: 2476478.

Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine.

Europe PMC ID: 2476478


Add a reference

References: 22 entries

Zilio et al. 2017. J Immunol. :. PMID: 28396317.

4PD Functionalized Dendrimers: A Flexible Tool for In Vivo Gene Silencing of Tumor-Educated Myeloid Cells.

Maternal obesity leads to increased proliferation and numbers of astrocytes in the developing fetal and neonatal mouse hypothalamus.

Kim et al. 2016. Int J Dev Neurosci. :. PMID: 27326907.

Yao et al. 2016. Sci Rep. 6:26050. PMID: 27198662.

IF

Functional assessment of glioma pathogenesis by in vivo multi-parametric magnetic resonance imaging and in vitro analyses.

Zhao et al. 2015. Oncogene. :. PMID: 26657156.

FACS

CBP/catenin antagonist safely eliminates drug-resistant leukemia-initiating cells.

Intratracheal cell transfer demonstrates the profibrotic potential of resident fibroblasts in pulmonary fibrosis.

Tsukui et al. 2015. Am J Pathol. :. PMID: 26456579.

Cui et al. 2015. Cell. 161(4):750-61. PMID: 25957683.

FACS

IL-7-Induced Glycerol Transport and TAG Synthesis Promotes Memory CD8+ T Cell Longevity.

Sheikh et al. 2015. Oncogene. PMID: 25772242.

FACS

MOZ (MYST3, KAT6A) inhibits senescence via the INK4A-ARF pathway.

Lin et al. 2013. Invest Ophthalmol Vis Sci. 54(3):1920-30. PMID: 23439595.

Involvement of SDF1a and STAT3 in granulocyte colony-stimulating factor rescues optic ischemia-induced retinal function loss by mobilizing hematopoietic stem cells.

Innis et al. 2010. Am J Physiol Gastrointest Liver Physiol. 299(6):G1376-85. PMID: 20864654.

Perinatal lipid nutrition alters early intestinal development and programs the response to experimental colitis in young adult rats.

Zindy et al. 2006. Proc Natl Acad Sci U S A. 103(31):11579-83. PMID: 16864777.

N-Myc and the cyclin-dependent kinase inhibitors p18Ink4c and p27Kip1 coordinately regulate cerebellar development.

Magaud et al. 1989. J Histochem Cytochem. 37(10):1517-27. PMID: 2476478.

Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine.


Add a reference


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