Anti-BrdU [BU20a] mAb

Tool Details

*FOR RESEARCH USE ONLY

• Name: Anti-BrdU [BU20a] mAb
• Clone: BU20a
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Host: Mouse
• Application: FACS ; IHC ; IF
• Description: Monoclonal antibody detects proliferating cells in the S phase via binding the synthetic nucleoside BrdU.
• Immunogen: Bromodeoxyuridine conjugated to BSA
• Isotype: IgG1
• Myeloma used: P3/NS1/1-Ag4.1

Target Details

• Target: Bromodeoxyuridine (BrdU)
• Target background: Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. It can be incorporated into the newly synthesized DNA of replicating cells, within the S phase of the cell cycle instead of thymidine. Anti-BrdU works well in immunohistochemistry to identify cells in tissue sections or cytospin preparations that have incorporated BrdU. This indicates cells that were actively replicating their DNA. Anti-BrdU stains BrdU incorporated into the nuclei of a wide range of cell types, including human tonsil lymphoid cells and human tumours growing in nude mice. It is also useful to identify cells in S phase during FACS analysis. Anti-BrdU can be used to analyse the phenotype of S-phase cells and in co-localizing antigen expression and BrdU incorporation in tissue sections. This antibody was created to replace similar use polyclonal antibodies which frequently cross reacted with other nucleotides. Binding of this antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

Applications

• Application: FACS ; IHC ; IF

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
• Shipping conditions: Shipping at 4° C

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