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Anti-PHD3 [EG188e/d5]


Catalogue Number 151315
Applications IHC WB
Antigen/Gene or Protein Targets Prolyl Hydroxylase 3 (PHD3)
Reactivity Human
Relevance PHD3 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins, hydroxylates HIF-1 alpha at Pro-564, and also hydroxylates HIF-2 alpha. It functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. It may play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. It promotes cell death through a caspase-dependent mechanism
Host Mouse
Immunogen Residues 1-100 of human PHD3
Subclass IgG1
Positive Control ZR75 cells on hypoxic induction
Molecular Weight (kDa) 27.3
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI + 10% FCS + 5% CO2
Strain Balb/c
Research Area Cancer, Cardiovascular, Epigenetics & Nuclear Signalling, Metabolism

References: 4 entries

Fujita et al. 2012. J Biol Chem. 287(47):39942-53. PMID: 22948157.

Soilleux et al. 2005. Histopathology. 47(6):602-10. PMID: 16324198.

Stolze et al. 2004. J Biol Chem. 279(41):42719-25. PMID: 15302861.

Appelhoff et al. 2004. J Biol Chem. 279(37):38458-65. PMID: 15247232.

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