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|Antigen/Gene or Protein Targets||Prolyl Hydroxylase 2 (PHD2)|
|Relevance||366G/76/3 recognises human prolyl hydroxylase 2 (PHD2), a 46kDa enzyme expressed abundantly in all tissues with the highest expression in testis. Hypoxia inducible factor-1 (HIF-1) is a transcriptional complex, consisting of an alpha and beta subunit, which plays a key role in coordinating the cellular response to hypoxia. During normal oxygen conditions, the alpha subunit of HIF-1 is rapidly degraded, however when hypoxia occurs this degradation is suppressed and HIF-1 activates the transcription of various genes important for survival and adaptation to hypoxia. Prolyl hydroxylase 2 catalyses the hydroxylation of specific prolyl residues within the HIF-1 alpha subunit, thereby targeting this subunit for degradation.|
|Immunogen||Residues 1-24 of PHD2|
|Positive Control||MCF7 cells|
|Molecular Weight (kDa)||46.1|
|Recommended Growing Conditions||RPMI + 10% FCS + 5% CO2|
|Research Area||Cancer, Cardiovascular, Epigenetics & Nuclear Signalling, Metabolism|
References: 4 entries
Jubb et al. 2009. Br J Cancer. 101(10):1749-57. PMID: 19844231.
Soilleux et al. 2005. Histopathology. 47(6):602-10. PMID: 16324198.
Stolze et al. 2004. J Biol Chem. 279(41):42719-25. PMID: 15302861.
Appelhoff et al. 2004. J Biol Chem. 279(37):38458-65. PMID: 15247232.
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