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Anti-PHD2 [366G/76/3]


Catalogue Number 151314
Applications IHC WB
Antigen/Gene or Protein Targets Prolyl Hydroxylase 2 (PHD2)
Reactivity Human
Relevance 366G/76/3 recognises human prolyl hydroxylase 2 (PHD2), a 46kDa enzyme expressed abundantly in all tissues with the highest expression in testis. Hypoxia inducible factor-1 (HIF-1) is a transcriptional complex, consisting of an alpha and beta subunit, which plays a key role in coordinating the cellular response to hypoxia. During normal oxygen conditions, the alpha subunit of HIF-1 is rapidly degraded, however when hypoxia occurs this degradation is suppressed and HIF-1 activates the transcription of various genes important for survival and adaptation to hypoxia. Prolyl hydroxylase 2 catalyses the hydroxylation of specific prolyl residues within the HIF-1 alpha subunit, thereby targeting this subunit for degradation.
Host Mouse
Immunogen Residues 1-24 of PHD2
Subclass IgG1
Positive Control MCF7 cells
Molecular Weight (kDa) 46.1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI + 10% FCS + 5% CO2
Strain Balb/c
Research Area Cancer, Cardiovascular, Epigenetics & Nuclear Signalling, Metabolism

References: 4 entries

Jubb et al. 2009. Br J Cancer. 101(10):1749-57. PMID: 19844231.

Soilleux et al. 2005. Histopathology. 47(6):602-10. PMID: 16324198.

Stolze et al. 2004. J Biol Chem. 279(41):42719-25. PMID: 15302861.

Appelhoff et al. 2004. J Biol Chem. 279(37):38458-65. PMID: 15247232.

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