Anti-Rad51B [rad51b 1h3/13]
Invented by Dr Stephen West from Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories
- Datasheet
- References (6)
- Inventor Info
Info
| Catalogue Number | 151268 |
| Applications | IP WB |
| Antigen/Gene or Protein Targets | Rad51B |
| Reactivity | Human |
| Relevance | RAD51B is a Rad51 paralog. RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. RAD51B is also a component for the homologous recombination pathway. |
| Host | Mouse |
| Immunogen | Rad51b 1h3/13 was raised against His-tagged human Rad51B, overexpressed in e.coli and purified on a talon affinity column under denaturing conditions, followed by gel purification on a SDS-PAGE. |
| Subclass | IgG2a |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Notes | Rad51b 1h3/13 detects as little as 10ng of purified protein |
| Research Area | DNA Damage and Repair, Epigenetics & Nuclear Signalling |
References: 6 entries
Chun et al. 2013. Mol Cell Biol. 33(2):387-95. PMID: 23149936.
Rad51 paralog complexes BCDX2 and CX3 act at different stages in the BRCA1-BRCA2-dependent homologous recombination pathway.
Europe PMC ID: 23149936
Masson et al. 2001. Genes Dev. 15(24):3296-307. PMID: 11751635.
Identification and purification of two distinct complexes containing the five RAD51 paralogs.
Europe PMC ID: 11751635
Albala et al. 1997. Genomics. 46(3):476-9. PMID: 9441753.
Identification of a novel human RAD51 homolog, RAD51B.
Europe PMC ID: 9441753
Add a reference
References: 6 entries
Chun et al. 2013. Mol Cell Biol. 33(2):387-95. PMID: 23149936.
Rad51 paralog complexes BCDX2 and CX3 act at different stages in the BRCA1-BRCA2-dependent homologous recombination pathway.
Masson et al. 2001. Genes Dev. 15(24):3296-307. PMID: 11751635.
Identification and purification of two distinct complexes containing the five RAD51 paralogs.
Albala et al. 1997. Genomics. 46(3):476-9. PMID: 9441753.
Identification of a novel human RAD51 homolog, RAD51B.
Add a reference
