Cat. #151268
Anti-Rad51B [rad51b 1h3/13]
Cat. #: 151268
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 1-2 weeks
Target: Rad51B
Class: Monoclonal
Application: IP ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Stephen West
Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
*FOR RESEARCH USE ONLY
- Name: Anti-Rad51B [rad51b 1h3/13]
- Clone: rad51b 1h3/13
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Strain: Balb/c
- Reactivity: Human
- Host: Mouse
- Application: IP ; WB
- Description: RAD51B is a Rad51 paralog. RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. RAD51B is also a component for the homologous recombination pathway.
- Immunogen: Rad51b 1h3/13 was raised against His-tagged human Rad51B, overexpressed in e.coli and purified on a talon affinity column under denaturing conditions, followed by gel purification on a SDS-PAGE.
- Isotype: IgG2a
- Myeloma used: Sp2/0-Ag14
Target Details
- Target: Rad51B
- Target background: RAD51B is a Rad51 paralog. RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase, and a component of the homologous recombination DNA repair pathway. RAD51 forms a nucleoprotein filament (through binding RAD52 and single stranded DNA that are exposed following double strand breaks) that initiates recombination. RAD51B is also a component for the homologous recombination pathway.
Applications
- Application: IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Chun et al. 2013. Mol Cell Biol. 33(2):387-95. PMID: 23149936.
- Rad51 paralog complexes BCDX2 and CX3 act at different stages in the BRCA1-BRCA2-dependent homologous recombination pathway.
- Masson et al. 2001. Genes Dev. 15(24):3296-307. PMID: 11751635.
- Identification and purification of two distinct complexes containing the five RAD51 paralogs.
- Albala et al. 1997. Genomics. 46(3):476-9. PMID: 9441753.
- Identification of a novel human RAD51 homolog, RAD51B.
