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Monoclonal Antibodies

Anti-BLM [BFL 103]

Invented by Helen Turley from University of Oxford
Invented at University of Oxford
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Chen et al. Molecular Medicine REPORTS 19: 4819-4831, 2019 Western blot analysis of BLM protein expression levels in PC3 cells at 24 h after miR or NC mimic transfection. NC, negative control; miR, microRNA; BLM, BLM RecQ like helicase.
Adapted from Verdun et al Cell 127, 709–720, November 17, 2006 Homologous Recombination Can Generate a D Loop Structure with Telomeric Sequences In Vitro. D loop assays were performed with nuclear extracts (IMR90 cells) previously depleted of the indicated proteins. The signals corresponding to the loop or free probe are indicated at the left.
From Turley et al British Journal of Cancer (2001) 85(2), 261–265 Staining of human normal and tumour tissues using the anti-BLM specific antibody, BFL-103. Panels A–D represent normal tissues, and panels E–H represent tumour tissue. Panels: A, kidney; B, placenta; C, tonsil, showing positive nuclei mainly in the germinal centre; D, thymus, showing extensive, but scattered positive nuclei; E, lymphoma of a large B-cell type; F, an example of a breast carcinoma, showing a high level of nuclear staining; G and H depict the same breast carcinoma sample stained with the BFL-103 and Ki67 antibodies, respectively. Note that a similar percentage of nuclei are stained positive in each case in panels G and H.
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Catalogue Number 151267
Applications ELISA IHC IF WB
Antigen/Gene or Protein Targets Bloom’s Syndrome Protein (BLM)
Reactivity Human
Relevance BLM is the product of the Blooms syndrome gene and belongs to the RecQ family of DNA helicases. BLM is associated with an increase in the incidence of many types of cancer at an early age.
Host Mouse
Immunogen Recombinant Full Length Bloom’s Protein
Positive Control Tonsil/thymus
Subclass IgG1
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions RPMI + 10% FCS + HAT
Strain Balb/c
Research Area Cancer

References

There are 7 reference entries for this reagent.

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References: 7 entries

Chen et al. 2019. Mol Med Rep. 19(6):4819-4831. PMID: 30957187.

WB

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Zhu et al. 2008. J Biol Chem. 283(43):29405-15. PMID: 18708356.

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Small ubiquitin-related modifier (SUMO) binding determines substrate recognition and paralog-selective SUMO modification.

Europe PMC ID: 18708356

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Turley et al. 2001. Br J Cancer. 85(2):261-5. PMID: 11461087.

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The distribution and expression of the Bloom's syndrome gene product in normal and neoplastic human cells.

Europe PMC ID: 11461087

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Wu et al. 2000. J Biol Chem. 275(13):9636-44. PMID: 10734115.

View  

The Bloom's syndrome gene product interacts with topoisomerase III.

Europe PMC ID: 10734115

View  

Add a reference

References: 7 entries

Chen et al. 2019. Mol Med Rep. 19(6):4819-4831. PMID: 30957187.

WB

View  

Zhu et al. 2008. J Biol Chem. 283(43):29405-15. PMID: 18708356.

View  

Small ubiquitin-related modifier (SUMO) binding determines substrate recognition and paralog-selective SUMO modification.

View  

Turley et al. 2001. Br J Cancer. 85(2):261-5. PMID: 11461087.

View  

The distribution and expression of the Bloom's syndrome gene product in normal and neoplastic human cells.

View  

Wu et al. 2000. J Biol Chem. 275(13):9636-44. PMID: 10734115.

View  

The Bloom's syndrome gene product interacts with topoisomerase III.

View  

Add a reference

Inventor Information