Anti-Cdc27 [AF 3.1]
Invented by Julian Gannon from The Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories
- Datasheet
- References (9)
- Inventor Info
Info
| Catalogue Number | 151232 |
| Applications | ELISA FACS IHC IP WB |
| Antigen/Gene or Protein Targets | Cell division cycle protein 27 (cdc27) |
| Synonyms | Cell Division Cycle 27; Anaphase Promoting Complex Subunit; D0S1430E; D17S978E; CDC27Hs; ANAPC3; H-NUC; APC3; Anaphase-Promoting Complex; Cell Division Cycle 27 Homolog; CDC27 Homolog; Nuc2 Homolog; HNUC; NUC2 |
| Reactivity | Mammalian and Xenopus laevis |
| Relevance | Cdc27 is a component of the anaphase promoting complex (APC) which is responsible for destroying proteins, including cyclins, which are involved in mitosis. AF 3.1 is recommended for the immunoprecipitation of active APC. |
| Host | Mouse |
| Immunogen | A peptide corresponding to the C-terminal 10 residues of human cdc27 coupled to KLH. |
| Positive Control | HeLa cells |
| Subclass | IgG2b |
| Molecular Weight (kDa) | 80 |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Research Area | Cell Cycle, Protein Degradation |
References: 9 entries
Mateo F et al. Oncogene. 2009 Jul 23;28(29):2654-66.
Ahlskog et al. 2010. Mol Cell Biol. 30(24):5608-20. PMID: 20937767.
Anaphase-promoting complex/cyclosome participates in the acute response to protein-damaging stress.
Europe PMC ID: 20937767
Holt et al. 2010. Development. 137(8):1297-304. PMID: 20223764.
Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes.
Europe PMC ID: 20223764
Hochegger et al. 2007. J Cell Biol. 178(2):257-68. PMID: 17635936.
An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells.
Europe PMC ID: 17635936
Yamano et al. 1998. EMBO J. 17(19):5670-8. PMID: 9755167.
The role of the destruction box and its neighbouring lysine residues in cyclin B for anaphase ubiquitin-dependent proteolysis in fission yeast: defining the D-box receptor.
Europe PMC ID: 9755167
Add a reference
References: 9 entries
Mateo F et al. Oncogene. 2009 Jul 23;28(29):2654-66.
Ahlskog et al. 2010. Mol Cell Biol. 30(24):5608-20. PMID: 20937767.
Anaphase-promoting complex/cyclosome participates in the acute response to protein-damaging stress.
Holt et al. 2010. Development. 137(8):1297-304. PMID: 20223764.
Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes.
Hochegger et al. 2007. J Cell Biol. 178(2):257-68. PMID: 17635936.
An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells.
Yamano et al. 1998. EMBO J. 17(19):5670-8. PMID: 9755167.
The role of the destruction box and its neighbouring lysine residues in cyclin B for anaphase ubiquitin-dependent proteolysis in fission yeast: defining the D-box receptor.
Add a reference
