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Anti-Cdc27 [AF 3.1]

Invented by Julian Gannon at The Francis Crick Institute

Info

Catalogue Number 151232
Applications ELISA FACS IHC IP WB
Antigen/Gene or Protein Targets Cell division cycle protein 27 (cdc27)
Synonyms Cell Division Cycle 27; Anaphase Promoting Complex Subunit; D0S1430E; D17S978E; CDC27Hs; ANAPC3; H-NUC; APC3; Anaphase-Promoting Complex; Cell Division Cycle 27 Homolog; CDC27 Homolog; Nuc2 Homolog; HNUC; NUC2
Reactivity Mammalian and Xenopus laevis
Relevance Cdc27 is a component of the anaphase promoting complex (APC) which is responsible for destroying proteins, including cyclins, which are involved in mitosis. AF 3.1 is recommended for the immunoprecipitation of active APC.
Host Mouse
Immunogen A peptide corresponding to the C-terminal 10 residues of human cdc27 coupled to KLH.
Subclass IgG2b
Positive Control HeLa cells
Molecular Weight (kDa) 80
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Research Area Cell Cycle, Protein Degradation

References: 9 entries

Mateo F et al. Oncogene. 2009 Jul 23;28(29):2654-66.

Ahlskog et al. 2010. Mol Cell Biol. 30(24):5608-20. PMID: 20937767.

Anaphase-promoting complex/cyclosome participates in the acute response to protein-damaging stress.

Europe PMC ID: 20937767

Holt et al. 2010. Development. 137(8):1297-304. PMID: 20223764.

Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes.

Europe PMC ID: 20223764

Hochegger et al. 2007. J Cell Biol. 178(2):257-68. PMID: 17635936.

An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells.

Europe PMC ID: 17635936

Yamano et al. 1998. EMBO J. 17(19):5670-8. PMID: 9755167.

The role of the destruction box and its neighbouring lysine residues in cyclin B for anaphase ubiquitin-dependent proteolysis in fission yeast: defining the D-box receptor.

Europe PMC ID: 9755167


Add a reference

References: 9 entries

Mateo F et al. Oncogene. 2009 Jul 23;28(29):2654-66.

Ahlskog et al. 2010. Mol Cell Biol. 30(24):5608-20. PMID: 20937767.

Anaphase-promoting complex/cyclosome participates in the acute response to protein-damaging stress.

Europe PMC ID: 20937767

Holt et al. 2010. Development. 137(8):1297-304. PMID: 20223764.

Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes.

Europe PMC ID: 20223764

Hochegger et al. 2007. J Cell Biol. 178(2):257-68. PMID: 17635936.

An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells.

Europe PMC ID: 17635936

Yamano et al. 1998. EMBO J. 17(19):5670-8. PMID: 9755167.

The role of the destruction box and its neighbouring lysine residues in cyclin B for anaphase ubiquitin-dependent proteolysis in fission yeast: defining the D-box receptor.

Europe PMC ID: 9755167


Add a reference

References: 9 entries

Mateo F et al. Oncogene. 2009 Jul 23;28(29):2654-66.

Ahlskog et al. 2010. Mol Cell Biol. 30(24):5608-20. PMID: 20937767.

Anaphase-promoting complex/cyclosome participates in the acute response to protein-damaging stress.

Holt et al. 2010. Development. 137(8):1297-304. PMID: 20223764.

Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes.

Hochegger et al. 2007. J Cell Biol. 178(2):257-68. PMID: 17635936.

An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells.

Yamano et al. 1998. EMBO J. 17(19):5670-8. PMID: 9755167.

The role of the destruction box and its neighbouring lysine residues in cyclin B for anaphase ubiquitin-dependent proteolysis in fission yeast: defining the D-box receptor.


Add a reference


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