Cat. #151232
Anti-Cdc27 [AF 3.1]
Cat. #: 151232
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 1-2 weeks
Target: Cell division cycle protein 27 (cdc27)
Class: Monoclonal
Application: ELISA ; FACS ; IHC ; IP ; WB
Reactivity: Mammalian ; Xenopus laevis
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Julian Gannon
Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
*FOR RESEARCH USE ONLY
- Name: Anti-Cdc27 [AF 3.1]
- Alternate name: Cell Division Cycle 27; Anaphase Promoting Complex Subunit; DS143E; D17S978E; CDC27Hs; ANAPC3; H-NUC; APC3; Anaphase-Promoting Complex; Cell Division Cycle 27 Homolog; CDC27 Homolog; Nuc2 Homolog; HNUC; NUC2
- Clone: AF 3.1
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: 80 kDa
- Strain: Balb/c
- Reactivity: Mammalian ; Xenopus laevis
- Host: Mouse
- Application: ELISA ; FACS ; IHC ; IP ; WB
- Description: AF 3.1 is recommended for the immunoprecipitation of active APC.
- Immunogen: A peptide corresponding to the C-terminal 10 residues of human cdc27 coupled to KLH.
- Isotype: IgG2b
- Myeloma used: Sp2/0-Ag14
- Recommended controls: HeLa cells
Target Details
- Target: Cell division cycle protein 27 (cdc27)
- Molecular weight: 80 kDa
- Tissue cell line specificity: HeLa cells
- Target background: Cdc27 is a component of the anaphase promoting complex (APC) which is responsible for destroying proteins, including cyclins, which are involved in mitosis.
Applications
- Application: ELISA ; FACS ; IHC ; IP ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Mateo F et al. Oncogene. 2009 Jul 23
- 28(29):2654-66.
- Ahlskog et al. 2010. Mol Cell Biol. 30(24):5608-20. PMID: 20937767.
- Anaphase-promoting complex/cyclosome participates in the acute response to protein-damaging stress.
- Holt et al. 2010. Development. 137(8):1297-304. PMID: 20223764.
- Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes.
- Hochegger et al. 2007. J Cell Biol. 178(2):257-68. PMID: 17635936.
- An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells.
- Yamano et al. 1998. EMBO J. 17(19):5670-8. PMID: 9755167.
- The role of the destruction box and its neighbouring lysine residues in cyclin B for anaphase ubiquitin-dependent proteolysis in fission yeast: defining the D-box receptor.
