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Anti-Integrin alpha 6 [MP4F10]
RECENTLY UPDATED

Info

Catalogue Number 151134
Applications
RECENTLY UPDATED
FACS IHC IF IP WB ICC
Antigen/Gene or Protein Targets Integrin alpha6 (CD49f)
Reactivity Human
Relevance Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin alpha 6 is found on epithelial and endothelial basement membranes and colorectal carcinoma. Integrin alpha 6 associates with integrin beta 4 to form integrin a6b4, one of the most abundant keratinocyte integrins, and binds laminin. MP 4F10 may be used in clinical studies for tumour grading as it will stain both moderately and well-differentiated colorectal adenocarcinoma.
Host Mouse
Immunogen SW1222 human colorectal cell line.
Subclass IgG1 kappa
Subclass Notes Inconclusive results
Myeloma Used P3/NS1/1-Ag4.1
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes This antibody has been tested and active in:
Inhibition of adhesion of human colon carcinoma cell line
avidin-biotin complex staining in human infant breast tissue
indirect immunoperoxidase staining in human colorectal carcinoma, human SW1222 cell line
GAG-ELISA of human type I collagen, type IV fibronectin and laminin, human colorectal adenocarcinoma

This antibody was tested in an indirect immunoperoxidase staining and found to NOT bind: human: intestine, placenta, skin, cervix, breast, endometrium, vagina, kidney, spleen, heart, smooth muscle and skeletal muscle
Research Area Adhesion, Developmental Biology, Stem Cell Biology

References

There are 11 reference entries for this reagent.

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References: 11 entries
RECENTLY UPDATED

Zhou et al. 2019. Stem Cell Res Ther. 10(1):155. PMID: 31151466.

Pieri et al. 2019. Stem Cell Res Ther. 10(1):135. PMID: 31109365.

Kapsokalyvas et al. 2017. Colloids Surf B Biointerfaces. 159:97-107. PMID: 28780465.

IHC

Zhang et al. 2017. PLoS One. 12(2):e0172207. PMID: 28234938.

Zhang et al. 2016. Cell Reprogram. 18(6):401-410. PMID: 27906584.

Al Thawadi et al. 2016. Oncotarget. 7(5):5289-305. PMID: 26700621.

WB

Stempin et al. 2015. Cancer Cell Int. 15:110. PMID: 26612978.

IF ICC

Svensson Månsson et al. 2007. J Pathol. 212(1):102-11. PMID: 17370299.

Levy et al. 2000. Mol Biol Cell. 11(2):453-66. PMID: 10679006.

Anbazhagan et al. 1995. J Pathol. 176(3):227-32. PMID: 7674085.

Pignatelli et al. 1990. Proc Natl Acad Sci U S A. 87(4):1541-5. PMID: 2304917.


Add a reference

References: 11 entries
RECENTLY UPDATED

Zhou et al. 2019. Stem Cell Res Ther. 10(1):155. PMID: 31151466.

Pieri et al. 2019. Stem Cell Res Ther. 10(1):135. PMID: 31109365.

Kapsokalyvas et al. 2017. Colloids Surf B Biointerfaces. 159:97-107. PMID: 28780465.

IHC

Zhang et al. 2017. PLoS One. 12(2):e0172207. PMID: 28234938.

Zhang et al. 2016. Cell Reprogram. 18(6):401-410. PMID: 27906584.

Al Thawadi et al. 2016. Oncotarget. 7(5):5289-305. PMID: 26700621.

WB

Stempin et al. 2015. Cancer Cell Int. 15:110. PMID: 26612978.

IF ICC

Svensson Månsson et al. 2007. J Pathol. 212(1):102-11. PMID: 17370299.

Levy et al. 2000. Mol Biol Cell. 11(2):453-66. PMID: 10679006.

Anbazhagan et al. 1995. J Pathol. 176(3):227-32. PMID: 7674085.

Pignatelli et al. 1990. Proc Natl Acad Sci U S A. 87(4):1541-5. PMID: 2304917.


Add a reference

Inventor Information