Cancer Research Technology
Log in Register
Menu

Info

Catalogue Number 151109
Applications FACS IHC IF Fn
Antigen/Gene or Protein Targets Integrin a3 (CD49c)
Reactivity Human
Relevance Monoclonal antibody with use studying inhibition of cellular adhesion.

Background and Research Application
Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. Integrin a3 is found on most cell types. Integrin a3 associates with b1 integrin to form integrin a3b1, which is one of the most abundant keratinocyte integrins and binds laminin 5.
IA3 is useful for studies on inhibition of cell-cell adhesion and cell-substrate adhesion.
Host Mouse
Immunogen Transfected human breast epithelial cell line MTSV1-7, hygromycin resistant.
Immunogen UniProt ID P26006
Subclass IgG1
Myeloma Used P3X63Ag8.653
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Concentration
1mg/ml as standard
Research Area Adhesion, Cancer

References

There are 12 reference entries for this reagent.

View All References

References: 12 entries

Tremblay et al. 2017. Sci Rep. 7:46574. PMID: 28422167.

Seltmann et al. 2015. J Invest Dermatol. 135(6):1609-1620. PMID: 25668239.

Seltmann et al. 2015. J Invest Dermatol. 135(6):1609-20. PMID: 25668239.

FACS IF

Keratins Stabilize Hemidesmosomes through Regulation of β4-Integrin Turnover.

Europe PMC ID: 25668239

Stawikowski et al. 2014. J Biol Chem. 289(31):21591-604. PMID: 24958723.

IF

Glycosylation modulates melanoma cell α2β1 and α3β1 integrin interactions with type IV collagen.

Europe PMC ID: 24958723

Pytlík et al. 2009. Biomaterials. 30(20):3415-27. PMID: 19362364.

FACS

The cultivation of human multipotent mesenchymal stromal cells in clinical grade medium for bone tissue engineering.

Europe PMC ID: 19362364

Turner et al. 2006. Circulation. 114(8):820-9. PMID: 16908762.

Alpha2(VIII) collagen substrata enhance endothelial cell retention under acute shear stress flow via an alpha2beta1 integrin-dependent mechanism: an in vitro and in vivo study.

Europe PMC ID: 16908762

Berdichevsky et al. 1994. J Cell Sci. 107 ( Pt 12):3557-68. PMID: 7535787.

Branching morphogenesis of human mammary epithelial cells in collagen gels.

Europe PMC ID: 7535787


Add a reference

References: 12 entries

Tremblay et al. 2017. Sci Rep. 7:46574. PMID: 28422167.

Seltmann et al. 2015. J Invest Dermatol. 135(6):1609-1620. PMID: 25668239.

Seltmann et al. 2015. J Invest Dermatol. 135(6):1609-20. PMID: 25668239.

FACS IF

Keratins Stabilize Hemidesmosomes through Regulation of β4-Integrin Turnover.

Stawikowski et al. 2014. J Biol Chem. 289(31):21591-604. PMID: 24958723.

IF

Glycosylation modulates melanoma cell α2β1 and α3β1 integrin interactions with type IV collagen.

Pytlík et al. 2009. Biomaterials. 30(20):3415-27. PMID: 19362364.

FACS

The cultivation of human multipotent mesenchymal stromal cells in clinical grade medium for bone tissue engineering.

Turner et al. 2006. Circulation. 114(8):820-9. PMID: 16908762.

Alpha2(VIII) collagen substrata enhance endothelial cell retention under acute shear stress flow via an alpha2beta1 integrin-dependent mechanism: an in vitro and in vivo study.

Berdichevsky et al. 1994. J Cell Sci. 107 ( Pt 12):3557-68. PMID: 7535787.

Branching morphogenesis of human mammary epithelial cells in collagen gels.


Add a reference