Anti-PTPRC [HLe-1(2D1)]
Invented by Prof Peter Beverley from Imperial College
Invented at Cancer Research UK London Research Institute: Lincoln's Inn Fields
- Datasheet
- References (7)
- Inventor Info
Info
| Catalogue Number | 151108 |
| Applications | FACS IHC IF |
| Antigen/Gene or Protein Targets | Protein tyrosine phosphatase, receptor type, C (PTPRC, CD45) |
| Reactivity | Human |
| Relevance |
Monoclonal antibody capable of analysing subpopulations of hematopoietic and lymphoid cells, and the degree of T cell differentiation. It can be used to identify the effect of CD45 in immune function. Background and Research Application Anti-PTPRC (Hle-1) monoclonal antibody binds transmembrane protein tyrosine phosphatase receptor type C (PTPRC), also known as CD45 antigen or leukocyte common antigen (LCA). Anti-PTPRC can be used in a panel of several monoclonal antibodies to characterise tumours. This antibody was created to investigate the similarities between normal thymocyte subpopulations and leukaemia’s of thymic origin (Thy-ALL), and aid with leukaemia diagnosis. PTPRC is present on all leukocytes. CD45 helps regulate T cell receptor-associated kinases and Janus kinases which transmit signals from cytokine receptors. This regulates the threshold of T cell antigen receptor (TCR) signalling through dephosphorylation of protein tyrosine kinases (e.g. Lck and Fyn). It is only expressed on nucleated hemopoietic cells (~10% of cell surface). CD45 phosphatase activity is required for efficient lymphocyte antigen receptor signal transduction. CD45 is a positive and negative regulator of Src family members, and can act as a JAK phosphatase, negatively regulating cytokine receptor activation (related to differentiation, proliferation and anti-viral immunity of hemopoietic cells). |
| Host | Mouse |
| Immunogen | Human peripheral blood mononuclear cells. |
| Immunogen UniProt ID | P08575 |
| Subclass | IgG1 |
| Molecular Weight (kDa) | 180-220 |
| Myeloma Used | P3/NS1/1-Ag4.1 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest Anti-PTPRC recognises all isoforms of CD45. It reacts strongly with cortical thymocytes, medullary thymus lymphocytes and peripheral lymphoid cells (including T and B cells), but reacts weakly with myeloid and erythroid bone marrow cells. Anti-PTPRC can be used to detect levels of T cell differentiation. The antibody clone name was called "Hle-1" in Cobbold et al., 1986. Proceedings of the 3rd International Workshop and Conference; Oxford, England. Oxford University Press; 1987. p. 788-803. |
| Research Area | Cardiovascular, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction, Immunology, Neurobiology, Stem Cell Biology |
References: 7 entries
Di Liberto et al. 2016. Clin Transl Gastroenterol. 7(7):e178. PMID: 27388423.
Lee et al. 2004. Int Immunol. 16(8):1109-24. PMID: 15210650.
Gene expression profiles during human CD4+ T cell differentiation.
Europe PMC ID: 15210650
Tchilian et al. 2001. J Immunol. 166(2):1308-13. PMID: 11145714.
A deletion in the gene encoding the CD45 antigen in a patient with SCID.
Europe PMC ID: 11145714
Bradstock et al. 1980. J Natl Cancer Inst. 65(1):33-42. PMID: 6993744.
Subpopulations of normal and leukemic human thymocytes: an analysis with the use of monoclonal antibodies.
Europe PMC ID: 6993744
Add a reference
References: 7 entries
Di Liberto et al. 2016. Clin Transl Gastroenterol. 7(7):e178. PMID: 27388423.
Lee et al. 2004. Int Immunol. 16(8):1109-24. PMID: 15210650.
Gene expression profiles during human CD4+ T cell differentiation.
Tchilian et al. 2001. J Immunol. 166(2):1308-13. PMID: 11145714.
A deletion in the gene encoding the CD45 antigen in a patient with SCID.
Bradstock et al. 1980. J Natl Cancer Inst. 65(1):33-42. PMID: 6993744.
Subpopulations of normal and leukemic human thymocytes: an analysis with the use of monoclonal antibodies.
Add a reference
