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Anti-cMyc [9E10]

Info

Catalogue Number 151067
Applications ChIP ELISA IHC IP WB
Antigen/Gene or Protein Targets c Myc epitope tag
Reactivity Human
Relevance Monoclonal antibody raised against sequence of c-Myc, a proto-oncogene heavily implicated in a variety of (hematopoietic) cancers, which has been used in cancer research to detect and understand the distribution of the c-Myc oncogene product.

Background and Research Application
Anti c-Myc is raised against the proto-oncogene c-Myc, analogous to the avian v-Myc gene a very strong proto-oncogene often found to be upregulated in cancer. Aberrant and constitutive expression of c-Myc has been particularly implicated in the upregulation of genes involved in cellular proliferation and has been identified in cancers including carcinoma of cervix, colon, breast, lung and stomach, plus a translocation leading to Burkitt’s lymphoma. The gene product of c-Myc (p62) is upregulated in various hematopoietic tumours.
9E10 recognises recombinant proteins incorporating a c-Myc epitope tag and detects human c-Myc protein as well as a peptide in random coil configuration not as a helix. It recognises overexpressed proteins containing the amino acid 408-439 sequence, of the human c-Myc gene product.

c-Myc is believed to regulate expression of 15% of all genes, hence its strong implicated in cancer and represents a promising therapeutic target. c-Myc has been shown to be involved in apoptosis, cell cycle progression, proliferation and differentiation. c-Myc carries out both its healthy and oncogenic functions within the nucleus. The c-Myc protein is a 62 kDa transcription factor encoded by c-Myc gene on human chromosome 8q24.

Anti c-Myc was created to precipitate p62 from human cells and recognise the c-Myc gene product, particularly in immunoblots to enable quantification of c-Myc in cells.
Host Mouse
Immunogen Synthetic peptide (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA - 32 aa) corresponding to 408-439 of Human c-Myc conjugated to KLH.
Immunogen UniProt ID P01106
Subclass IgG1 kappa
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Strain Balb/c
Notes Production Details
Purified using multi-step affinity chromatography with protein A.

Storage Conditions
Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles.

Points of Interest
Anti c-Myc 9E10 is the most sensitive probe out of all 6 created at the same time. It can be used to facilitate research into function pathology of c-Myc expression in normal and neoplastic cells. Anti c-Myc antibody has be used in protein localisation, immunohistochemistry, ELISA and protein purification, though identification of c-Myc tagged proteins.
The epitope recognised by anti c-Myc, comprising the amino acid sequence `Glu‐Gln‐Lys‐Leu‐Ile‐Ser‐Glu‐Glu‐Asp‐Leu‐Asn', can be expressed in a different protein context and still confers recognition by the anti c-Myc 9E10 antibody.

Concentration
1mg/ml as standard

Difference Between Clones
Anti c-Myc (9E11) – directed against residues 408-420 (AEEQKLISEEDL), which is slightly shorter than 9E10 and with specificity for IgG2a.
Anti c-Myc recombinant (9E10). This is a recombinant version of the original anti c-Myc.
K14MycER mouse model with c-Myc2 cDNA fused to hormone binding domain of a mutant mouse estragen receptor (ER).
Research Area Cancer, Cell Cycle, Epigenetics & Nuclear Signalling, Stem Cell Biology

References

There are 20 reference entries for this reagent.

View All References

References: 20 entries

Si et al. 2018. Int J Biol Sci. 14(2):165-177. PMID: 29483835.

Oh et al. 2018. Cell Rep. 25(7):1681-1692.e4. PMID: 30428339.

Aldon et al. 2018. Cell Rep. 24(12):3324-3338.e5. PMID: 30232012.

Rodríguez-Escudero et al. 2018. Sci Rep. 8(1):7732. PMID: 29769614.

Mateos-Gomez et al. 2015. Nature. 518(7538):254-7. PMID: 25642960.

WB

Mammalian polymerase θ promotes alternative NHEJ and suppresses recombination.

Europe PMC ID: 25642960

Zhang et al. 2014. Genes Dev. 28(8):829-34. PMID: 24736842.

ChIP

The diabetes gene Hhex maintains δ-cell differentiation and islet function.

Europe PMC ID: 24736842

Zhou et al. 2012. J Cell Biol. 196(2):203-11. PMID: 22270916.

IP WB

Novel plant SUN-KASH bridges are involved in RanGAP anchoring and nuclear shape determination.

Europe PMC ID: 22270916

Mendes et al. 2010. J Biol Chem. 285(50):39117-26. PMID: 20937822.

IP WB

Protein kinase WNK1 promotes cell surface expression of glucose transporter GLUT1 by regulating a Tre-2/USP6-BUB2-Cdc16 domain family member 4 (TBC1D4)-Rab8A complex.

Europe PMC ID: 20937822

Royds et al. 1992. J Pathol. 166(3):225-33. PMID: 1381423.

IHC

Cellular localisation of c-myc product in human colorectal epithelial neoplasia.

Europe PMC ID: 1381423

Spandidos et al. 1987. Anticancer Res. 7(6):1299-304. PMID: 3327455.

IHC

Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue.

Europe PMC ID: 3327455

Kari et al. 1986. J Virol. 60(2):345-52. PMID: 3021969.

IP

Characterization of monoclonal antibodies reactive to several biochemically distinct human cytomegalovirus glycoprotein complexes.

Europe PMC ID: 3021969

Evan et al. 1985. Mol Cell Biol. 5(12):3610-6. PMID: 3915782.

WB

Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.

Europe PMC ID: 3915782


Add a reference

References: 20 entries

Si et al. 2018. Int J Biol Sci. 14(2):165-177. PMID: 29483835.

Oh et al. 2018. Cell Rep. 25(7):1681-1692.e4. PMID: 30428339.

Aldon et al. 2018. Cell Rep. 24(12):3324-3338.e5. PMID: 30232012.

Rodríguez-Escudero et al. 2018. Sci Rep. 8(1):7732. PMID: 29769614.

Mateos-Gomez et al. 2015. Nature. 518(7538):254-7. PMID: 25642960.

WB

Mammalian polymerase θ promotes alternative NHEJ and suppresses recombination.

Zhang et al. 2014. Genes Dev. 28(8):829-34. PMID: 24736842.

ChIP

The diabetes gene Hhex maintains δ-cell differentiation and islet function.

Zhou et al. 2012. J Cell Biol. 196(2):203-11. PMID: 22270916.

IP WB

Novel plant SUN-KASH bridges are involved in RanGAP anchoring and nuclear shape determination.

Mendes et al. 2010. J Biol Chem. 285(50):39117-26. PMID: 20937822.

IP WB

Protein kinase WNK1 promotes cell surface expression of glucose transporter GLUT1 by regulating a Tre-2/USP6-BUB2-Cdc16 domain family member 4 (TBC1D4)-Rab8A complex.

Royds et al. 1992. J Pathol. 166(3):225-33. PMID: 1381423.

IHC

Cellular localisation of c-myc product in human colorectal epithelial neoplasia.

Spandidos et al. 1987. Anticancer Res. 7(6):1299-304. PMID: 3327455.

IHC

Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue.

Kari et al. 1986. J Virol. 60(2):345-52. PMID: 3021969.

IP

Characterization of monoclonal antibodies reactive to several biochemically distinct human cytomegalovirus glycoprotein complexes.

Evan et al. 1985. Mol Cell Biol. 5(12):3610-6. PMID: 3915782.

WB

Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.


Add a reference

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