| Catalogue Number | 151067 |
| Applications | ChIP ELISA IHC IP WB |
| Antigen/Gene or Protein Targets | c Myc epitope tag |
| Reactivity | Human |
| Relevance |
Monoclonal antibody raised against sequence of c-Myc, a proto-oncogene heavily implicated in a variety of (hematopoietic) cancers, which has been used in cancer research to detect and understand the distribution of the c-Myc oncogene product. Background and Research Application Anti c-Myc is raised against the proto-oncogene c-Myc, analogous to the avian v-Myc gene a very strong proto-oncogene often found to be upregulated in cancer. Aberrant and constitutive expression of c-Myc has been particularly implicated in the upregulation of genes involved in cellular proliferation and has been identified in cancers including carcinoma of cervix, colon, breast, lung and stomach, plus a translocation leading to Burkitt’s lymphoma. The gene product of c-Myc (p62) is upregulated in various hematopoietic tumours. 9E10 recognises recombinant proteins incorporating a c-Myc epitope tag and detects human c-Myc protein as well as a peptide in random coil configuration not as a helix. It recognises overexpressed proteins containing the amino acid 408-439 sequence, of the human c-Myc gene product. c-Myc is believed to regulate expression of 15% of all genes, hence its strong implicated in cancer and represents a promising therapeutic target. c-Myc has been shown to be involved in apoptosis, cell cycle progression, proliferation and differentiation. c-Myc carries out both its healthy and oncogenic functions within the nucleus. The c-Myc protein is a 62 kDa transcription factor encoded by c-Myc gene on human chromosome 8q24. Anti c-Myc was created to precipitate p62 from human cells and recognise the c-Myc gene product, particularly in immunoblots to enable quantification of c-Myc in cells. |
| Host | Mouse |
| Immunogen | Synthetic peptide (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA - 32 aa) corresponding to 408-439 of Human c-Myc conjugated to KLH. |
| Immunogen UniProt ID | P01106 |
| Subclass | IgG1 kappa |
| Myeloma Used | Sp2/0-Ag14 |
| Recommended Growing Conditions | DMEM + 5% FCS |
| Strain | Balb/c |
| Notes |
Production Details Purified using multi-step affinity chromatography with protein A. Storage Conditions Store at -20 degrees frozen. Avoid repeated freeze/thaw cycles. Points of Interest Anti c-Myc 9E10 is the most sensitive probe out of all 6 created at the same time. It can be used to facilitate research into function pathology of c-Myc expression in normal and neoplastic cells. Anti c-Myc antibody has be used in protein localisation, immunohistochemistry, ELISA and protein purification, though identification of c-Myc tagged proteins. The epitope recognised by anti c-Myc, comprising the amino acid sequence `Glu‐Gln‐Lys‐Leu‐Ile‐Ser‐Glu‐Glu‐Asp‐Leu‐Asn', can be expressed in a different protein context and still confers recognition by the anti c-Myc 9E10 antibody. Concentration 1mg/ml as standard Difference Between Clones Anti c-Myc (9E11) – directed against residues 408-420 (AEEQKLISEEDL), which is slightly shorter than 9E10 and with specificity for IgG2a. Anti c-Myc recombinant (9E10). This is a recombinant version of the original anti c-Myc. K14MycER mouse model with c-Myc2 cDNA fused to hormone binding domain of a mutant mouse estragen receptor (ER). |
| Research Area | Cancer, Cell Cycle, Epigenetics & Nuclear Signalling, Stem Cell Biology |
Invented at Absolute Antibody University of California, San Francisco
Si et al. 2018. Int J Biol Sci. 14(2):165-177. PMID: 29483835.
Oh et al. 2018. Cell Rep. 25(7):1681-1692.e4. PMID: 30428339.
Aldon et al. 2018. Cell Rep. 24(12):3324-3338.e5. PMID: 30232012.
Rodríguez-Escudero et al. 2018. Sci Rep. 8(1):7732. PMID: 29769614.
Mateos-Gomez et al. 2015. Nature. 518(7538):254-7. PMID: 25642960.
WB
Mammalian polymerase θ promotes alternative NHEJ and suppresses recombination.
Europe PMC ID: 25642960
Zhang et al. 2014. Genes Dev. 28(8):829-34. PMID: 24736842.
ChIP
The diabetes gene Hhex maintains δ-cell differentiation and islet function.
Europe PMC ID: 24736842
Zhou et al. 2012. J Cell Biol. 196(2):203-11. PMID: 22270916.
IP WB
Novel plant SUN-KASH bridges are involved in RanGAP anchoring and nuclear shape determination.
Europe PMC ID: 22270916
Mendes et al. 2010. J Biol Chem. 285(50):39117-26. PMID: 20937822.
IP WB
Protein kinase WNK1 promotes cell surface expression of glucose transporter GLUT1 by regulating a Tre-2/USP6-BUB2-Cdc16 domain family member 4 (TBC1D4)-Rab8A complex.
Europe PMC ID: 20937822
Royds et al. 1992. J Pathol. 166(3):225-33. PMID: 1381423.
IHC
Cellular localisation of c-myc product in human colorectal epithelial neoplasia.
Europe PMC ID: 1381423
Spandidos et al. 1987. Anticancer Res. 7(6):1299-304. PMID: 3327455.
IHC
Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue.
Europe PMC ID: 3327455
Kari et al. 1986. J Virol. 60(2):345-52. PMID: 3021969.
IP
Characterization of monoclonal antibodies reactive to several biochemically distinct human cytomegalovirus glycoprotein complexes.
Europe PMC ID: 3021969
Evan et al. 1985. Mol Cell Biol. 5(12):3610-6. PMID: 3915782.
WB
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
Europe PMC ID: 3915782
Si et al. 2018. Int J Biol Sci. 14(2):165-177. PMID: 29483835.
Oh et al. 2018. Cell Rep. 25(7):1681-1692.e4. PMID: 30428339.
Aldon et al. 2018. Cell Rep. 24(12):3324-3338.e5. PMID: 30232012.
Rodríguez-Escudero et al. 2018. Sci Rep. 8(1):7732. PMID: 29769614.
Mateos-Gomez et al. 2015. Nature. 518(7538):254-7. PMID: 25642960.
WB
Mammalian polymerase θ promotes alternative NHEJ and suppresses recombination.
Zhang et al. 2014. Genes Dev. 28(8):829-34. PMID: 24736842.
ChIP
The diabetes gene Hhex maintains δ-cell differentiation and islet function.
Zhou et al. 2012. J Cell Biol. 196(2):203-11. PMID: 22270916.
IP WB
Novel plant SUN-KASH bridges are involved in RanGAP anchoring and nuclear shape determination.
Mendes et al. 2010. J Biol Chem. 285(50):39117-26. PMID: 20937822.
IP WB
Protein kinase WNK1 promotes cell surface expression of glucose transporter GLUT1 by regulating a Tre-2/USP6-BUB2-Cdc16 domain family member 4 (TBC1D4)-Rab8A complex.
Royds et al. 1992. J Pathol. 166(3):225-33. PMID: 1381423.
IHC
Cellular localisation of c-myc product in human colorectal epithelial neoplasia.
Spandidos et al. 1987. Anticancer Res. 7(6):1299-304. PMID: 3327455.
IHC
Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue.
Kari et al. 1986. J Virol. 60(2):345-52. PMID: 3021969.
IP
Characterization of monoclonal antibodies reactive to several biochemically distinct human cytomegalovirus glycoprotein complexes.
Evan et al. 1985. Mol Cell Biol. 5(12):3610-6. PMID: 3915782.
WB
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
