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Monoclonal Antibodies

Anti-MBP [R29.6]

Invented by Julian Gannon from The Francis Crick Institute
Invented at Cancer Research UK London Research Institute: Clare Hall Laboratories
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Adapted from Verhoeven et al. 2009. PLoS One. 4(8):e6739. PMID: 19707582 The 17 kDa OmpA degradation band fractionates to the soluble periplasmic fraction. Soluble periplasmic fractions (s) and insoluble cell pellet (i) were prepared as described in Supplementary Materials and Methods. Shown are immunoblots of the strain MC1061ΔOmpA containing constructs expressing full-length OmpA without tag insertion (pGI9) and full length OmpA with a 3xFLAG inserted in Loop 2 (pGV32). The latter protein is present at the expected height in the insoluble fraction, as expected for a properly assembled OM protein. The stronger 17 kDa degradation band is present in the soluble periplasmic fraction instead. Only the relevant portions of the blot are shown. The soluble periplasmic protein MBP (40 kDa), inducible by growth on maltose (0.2%), was used as a fractionation marker. The polyclonal anti-OmpA antibody was used in a 1∶10000 dilution (upper blot) and the monoclonal anti-MBP antibody (Abcam, #ab65) was used at a concentration of 1.8 µg/ml (lower blot).
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Catalogue Number 151017
Applications ChIP IHC IF IP WB
Antigen/Gene or Protein Targets Maltose binding protein (MBP)
Reactivity N/A
Relevance MBP is a bacterial protein commonly used as a fusion protein. R29.6 is useful for detection and isolation of recombinant MBP fusion proteins.
Host Mouse
Immunogen MOS maltose binding protein fusion protein
Positive Control MBP fusion protein generated with the pmal plasmid (New England Biolabs) in bacterial lysate.
Subclass IgG1
Molecular Weight (kDa) 53
Myeloma Used Sp2/0-Ag14
Recommended Growing Conditions DMEM + 5% FCS
Research Area Adhesion, Cell Type or Organelle Marker, Neurobiology

References

There are 8 reference entries for this reagent.

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References: 8 entries

Verhoeven et al. 2009. PLoS One. 4(8):e6739. PMID: 19707582.

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Differential bacterial surface display of peptides by the transmembrane domain of OmpA.

Europe PMC ID: 19707582

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Im et al. 2009. Dev Cell. 17(2):234-43. PMID: 19686684.

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Structure and function of the ESCRT-II-III interface in multivesicular body biogenesis.

Europe PMC ID: 19686684

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Liu et al. 2006. Genome Res. 16(12):1517-28. PMID: 17053089.

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Whole-genome comparison of Leu3 binding in vitro and in vivo reveals the importance of nucleosome occupancy in target site selection.

Europe PMC ID: 17053089

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Liu et al. 2005. Genome Res. 15(3):421-7. PMID: 15710749.

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DIP-chip: rapid and accurate determination of DNA-binding specificity.

Europe PMC ID: 15710749

View  

Add a reference

References: 8 entries

Verhoeven et al. 2009. PLoS One. 4(8):e6739. PMID: 19707582.

View  

Differential bacterial surface display of peptides by the transmembrane domain of OmpA.

View  

Im et al. 2009. Dev Cell. 17(2):234-43. PMID: 19686684.

View  

Structure and function of the ESCRT-II-III interface in multivesicular body biogenesis.

View  

Liu et al. 2006. Genome Res. 16(12):1517-28. PMID: 17053089.

View  

Whole-genome comparison of Leu3 binding in vitro and in vivo reveals the importance of nucleosome occupancy in target site selection.

View  

Liu et al. 2005. Genome Res. 15(3):421-7. PMID: 15710749.

View  

DIP-chip: rapid and accurate determination of DNA-binding specificity.

View  

Add a reference

Inventor Information