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MLL-AF9-miR-139 knock out cell line

Invented by Stefan Erkeland
Invented at Erasmus Medical Center
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A Flow cytometric analysis of surface marker expression of MLL-AF9 or MLL-AF9-Mir139KO cells stained for myeloid lineage markers CD11b, CD16/32, c-Kit, GR-1 and lymphoid lineage marker CD3. B Heatmap showing the differential gene expression profiles of WT HSPCs (WT), Mir139KO HSPCs (KO), and MLL-AF9 cells (MA9). C The number of colony-forming units (CFUs) per 5000 empty vector control (−) and MLL-AF9 (MA9) transduced WT and Mir139KO HSPCs are shown. Total number of colonies (50–250 cells/colony) and large colonies (>250 cells/colony) are presented. Micrographs depict representative colonies. Scale bar indicates 100 µm. The two-tailed unpaired student’s t test was used for the statistical analysis. D Expression of miR-139-5p and miR-139-3p in MA9 cells and MLL-AF9-Mir139KO relative to snRNA U6 and EV transduced HSPCs (EV) as determined by miRNA qPCR is shown. The two-tailed unpaired student’s t test with Welch’s correction was used for the statistical analysis. The presented data are representative of two experiments. E Expression of Pde2a in MA9 cells and WT HSPCs in fragments per kilobase and per million mapped fragments (FPKM) as determined by RNA-seq is shown. The Wald’s test with Benjamin–Hochberg correction was used for the statistical analysis. All graphs show mean ± SEM. Adapted from Stavast, C.J., van Zuijen, I., Karkoulia, E. et al. The tumor suppressor MIR139 is silenced by POLR2M to promote AML oncogenesis. Leukemia 36, 687–700 (2022). https://doi.org/10.1038/s41375-021-01461-5
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Catalogue Number 160880
Antigen/Gene or Protein Targets MIR139
Parental Line Lineage negative bone marrow cells isolated from miR139 KO mice
Synonyms miR-139
Host Mouse
Tissue Bone Marrow
Disease Keywords Acute Myeloid Leukaemia (AML)
Model Cancer Model
Relevance MIR-139 is a critical tumor surpressor in AML
Production Details Lineage negative bone marrow cells were isolated from miR139 KO mice and transduced with MLL-AF9.
Conditional No
Research Area Cancer
Growth/Phenotype Keywords Enhanced Proliferation
Recommended Growing Conditions As published in Stavast, C.J., van Zuijen, I., Karkoulia, E. et al. The tumor suppressor MIR139 is silenced by POLR2M to promote AML oncogenesis. Leukemia 36, 687–700 (2022). https://doi.org/10.1038/s41375-021-01461-5
Positive Control MIR139 WT, MLL-AF9 cells

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References: 1 entry

Stavast et al. 2022. Leukemia. 36(3):687-700. PMID: 34741119.

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References: 1 entry

Stavast et al. 2022. Leukemia. 36(3):687-700. PMID: 34741119.

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