IL-17A (MM17F3)
Invented by Uyttenhove Catherine, Van Snick Jacques
Invented at Ludwig Institute for Cancer Research
- Datasheet
- References (1)
- Inventor Info
Info
| Catalogue Number | 160874 |
| Applications | ELISA |
| Antigen/Gene or Protein Targets | Interleukin-17A; IL17A; Cytotoxic T-lymphocyte-associated antigen 8; CTLA-8 |
| Synonyms | Interleukin-17A; IL17A |
| Reactivity | N/A |
| Relevance | Blocking, therapeutic, ELISA |
| Host | Mouse |
| Recommended Growing Conditions | 3T3 based IL-17A/F bioassay 3T3 seeding Celles are seeded in 48w plates, 30 000 cells/w in 250ul of DMEM 10% FCS Cells are incubated 24h 37° 5% CO2 |
| Notes |
Treatment of the cells Discard the culture medium and replace by 100 ul medium containing -30ng /ml murine IL-17A -100 ng murine IL-17F If you want to test inhibition activity of mAbs, préincubate a mixture of IL-17 and serial dilutions of the mAbs ( from 1000ng/ml to ± 1 ng/ml) in separated wells (min 30 min, usually around 4H) before adding to the 3T3 cells. Incubate the cells + mAbs + IL17 min 24H, preferably up to 40H. Collect SN from each well and store frozen till IL-6 Elisa. IL-6 ELISA – dilute SN 1/10 to perform the murine IL-6 ELISA. PS. Some FCS batches induce rapidly a shift in the 3T3 cells that become either insensitive to IL-17 or stop IL-6 secretion in response. Prepare quite a large batch of frozen vials as soon as possible. |
| Research Area | Immunology |
References: 1 entry
References: 1 entry
Inventor Information
Inventors
|
Uyttenhove Catherine, Van Snick Jacques |
