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IL-17A (MM17F3)

Invented by Uyttenhove Catherine, Van Snick Jacques
Invented at Ludwig Institute for Cancer Research

Info

Catalogue Number 160874
Applications ELISA
Antigen/Gene or Protein Targets Interleukin-17A; IL17A; Cytotoxic T-lymphocyte-associated antigen 8; CTLA-8
Synonyms Interleukin-17A; IL17A
Reactivity N/A
Relevance Blocking, therapeutic, ELISA
Host Mouse
Recommended Growing Conditions 3T3 based IL-17A/F bioassay 3T3 seeding Celles are seeded in 48w plates, 30 000 cells/w in 250ul of DMEM 10% FCS Cells are incubated 24h 37° 5% CO2
Notes Treatment of the cells
Discard the culture medium and replace by 100 ul medium containing
-30ng /ml murine IL-17A
-100 ng murine IL-17F
If you want to test inhibition activity of mAbs, préincubate a mixture of IL-17 and serial dilutions of the mAbs ( from 1000ng/ml to ± 1 ng/ml) in separated wells (min 30 min, usually around 4H) before adding to the 3T3 cells.

Incubate the cells + mAbs + IL17 min 24H, preferably up to 40H. Collect SN from each well and store frozen till IL-6 Elisa.
IL-6 ELISA – dilute SN 1/10 to perform the murine IL-6 ELISA.
PS. Some FCS batches induce rapidly a shift in the 3T3 cells that become either insensitive to IL-17 or stop IL-6 secretion in response. Prepare quite a large batch of frozen vials as soon as possible.
Research Area Immunology

References

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References: 1 entry

Uyttenhove et al. 2006. Eur J Immunol. 36(11):2868-74. PMID: 17048276.


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References: 1 entry

Uyttenhove et al. 2006. Eur J Immunol. 36(11):2868-74. PMID: 17048276.


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Inventor Information

Inventors

Uyttenhove Catherine, Van Snick Jacques

Uyttenhove Catherine, Van Snick Jacques

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