PNPLA1-flox mouse

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Info

Catalogue Number	160872
Antigen/Gene or Protein Targets	patatin-like phospholipase domain containing 1, ichthyosis, ARCI
Synonyms	PNPLA1
Model	Conditional KO
Relevance	Useful model for the generation of tissue/cell-specific PNPLA1-deficient mice. Animals might be useful for studies, e.g. on skin development/barrier function or omega-O-acylceramide metabolism, or as model for Autosomal Recessive Congenital Ichthyosis (ARCI).
Production Details	A floxed neomycin resistance cassette was inserted upstream of exon 1. An additional loxP site was inserted downstream of exon 1. Cre-mediated recombination removed the selection cassette and left Pnpla1 exon 1 floxed. Further details are available upon request.
Breeding Information	Good breeder. Homozygous mice may be bred together to maintain a live colony,.
Conditional	Yes
Conditional Description	Targeted conditional ready
Growth/Phenotype Keywords	These mice are conditional ready, and phenotypical identical to C57BL/6J mice.
Strain	C57BL/6J
Mouse Genetic Background/Cross History	These mice were generated using HM1-ES cells. ES cell clones were injected into C57BL/6J blastocysts to enable coat colour selection of the chimeras. Mice were subsequently backcrossed to C57BL/6J (≥ 10 generations). When kept as a homozygous colony, mice are regularly backcrossed to C57BL/6J to avoid generation of subpopulations.
Zygosity	Homozygous
Research Area	Metabolism, Cell Signaling & Signal Transduction

There are 1 reference entries for this reagent.

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Grond et al. 2017. J Invest Dermatol. 137(2):394-402. PMID: 27751867.

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Grond et al. 2017. J Invest Dermatol. 137(2):394-402. PMID: 27751867.

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Franz Radner

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	University of Graz
	11 reagents listed on Ximbio

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