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Adenovirus ALDH2 double mutant (human; mitochondrial; C301S/C303S) vector

Invented by Bernhard-Michael Mayer
Invented at University of Graz
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Image thumbnail for Adenovirus ALDH2 double mutant (human; mitochondrial; C301S/C303S) vector
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ALDH2 expression in non-infected and infected vascular smooth muscle and porcine aortic endothelial cells. A, expression of ALDH2 in non-infected and infected (WT or C301S/C303S ALDH2) VSMC (n = 4). B, representative Western blot of total homogenates of vascular smooth muscle cells (10 μg of protein), showing ALDH2 (54 kDa) and β-actin (43 kDa). Purified human ALDH2 (25 ng) was used as standard (S) for protein quantification. C, expression of ALDH2 in non-infected and infected (WT or C301S/C303S ALDH2) porcine aortic endothelial cells (PAEC) (n = 5). D, representative Western blot of total homogenates of porcine endothelial cells (10 μg of protein), showing ALDH2 (54 kDa) and β-actin (43 kDa). Purified human ALDH2 (25 ng) was used as standard (S) for protein quantification. Summary data are expressed as ng of ALDH2/μg of total protein and represent mean values ± S.E. of the number of experiments indicated in the panel description above. Adapted from Opelt M, Eroglu E, Waldeck-Weiermair M, et al. Formation of Nitric Oxide by Aldehyde Dehydrogenase-2 Is Necessary and Sufficient for Vascular Bioactivation of Nitroglycerin. J Biol Chem. 2016;291(46):24076-24084. doi:10.1074/jbc.M116.752071
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Image thumbnail for Adenovirus ALDH2 double mutant (human; mitochondrial; C301S/C303S) vector
Catalogue Number 160859
Backbone Size (bp) 33414
Bacterial Resistance Ampicillin
Vector Type pAdEasy-1
Antigen/Gene or Protein Targets Aldehyde dehydrogenase 2, mictochondrial (NCBI; Gene ID:217)
Relevance This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism.
Research Area Cardiovascular

References

There are 2 reference entries for this reagent.

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References: 2 entries

Opelt et al. 2016. J Biol Chem. 291(46):24076-24084. PMID: 27679490.

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Wenzl et al. 2011. Mol Pharmacol. 80(2):258-66. PMID: 21536753.

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References: 2 entries

Opelt et al. 2016. J Biol Chem. 291(46):24076-24084. PMID: 27679490.

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Wenzl et al. 2011. Mol Pharmacol. 80(2):258-66. PMID: 21536753.

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Inventor Information

Inventors

Bernhard-Michael Mayer

Bernhard-Michael Mayer

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Invented at

University of Graz
University of Graz

11 reagents listed on Ximbio