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SARS-CoV-2 RBD fragment cell line

Invented at The University of Minnesota, Twin Cities

Info

Catalogue Number 160855
Antigen/Gene or Protein Targets Antigen, protein fragment, receptor binding domain
Parental Line HEK293F
Synonyms Recombinant SARS-CoV-2 RBD
Host Human
Disease Keywords Coronavirus, COVID-19, SARS-CoV-2
Relevance The SAR-CoV-2 RBD fragment expressed by this cell line has been successfully used to make ELISA assays for detection of coronavirus infection in human patients.
Production Details To constitutively express SARS-CoV-2 RBD, HEK293F cell line was genetically modified in the lab using the lentivirus system. More specifically, the SARS-CoV-2 RBD gene was inserted into the genome of HEK293F cells using lentiviruses, and the stably transfected cells constitutively secrete SARS-CoV-2 RBD into the cell medium at high levels. The RBD fragment has 8X his tags for subsequent purification.
Research Area Drug Discovery & Development, Immunology, Structural Biology, Virology
Recommended Growing Conditions The cells are cultured in FreeStyle 293 Expression Medium from Thermo Fisher.
Positive Control No

References

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References: 1 entry

Shang et al. 2020. Nature. 581(7807):221-224. PMID: 32225175.


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References: 1 entry

Shang et al. 2020. Nature. 581(7807):221-224. PMID: 32225175.


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