Catalogue Number
|
160855 |
Antigen/Gene or Protein Targets
|
Antigen, protein fragment, receptor binding domain
|
Parental Line
|
HEK293F
|
Synonyms
|
Recombinant SARS-CoV-2 RBD
|
Host
|
Human
|
Disease Keywords
|
Coronavirus, COVID-19, SARS-CoV-2
|
Relevance
|
The SAR-CoV-2 RBD fragment expressed by this cell line has been successfully used to make ELISA assays for detection of coronavirus infection in human patients.
|
Production Details
|
To constitutively express SARS-CoV-2 RBD, HEK293F cell line was genetically modified in the lab using the lentivirus system. More specifically, the SARS-CoV-2 RBD gene was inserted into the genome of HEK293F cells using lentiviruses, and the stably transfected cells constitutively secrete SARS-CoV-2 RBD into the cell medium at high levels. The RBD fragment has 8X his tags for subsequent purification.
|
Research Area
|
Drug Discovery & Development, Immunology, Structural Biology, Virology
|
Recommended Growing Conditions
|
The cells are cultured in FreeStyle 293 Expression Medium from Thermo Fisher.
|
Positive Control
|
No
|