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CHO DC-SIGN-N80A cell line

Invented by Alessandra Cambi
Invented at Radboud UMC

Info

Catalogue Number 160717
Antigen/Gene or Protein Targets Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin lacking the ligand binding domain N80.
Parental Line CHO
Synonyms DC-SIGN N80A
Host Hamster
Tissue Ovary
Disease Keywords HIV-1, Ebola Virus, Hepatitis C virus
Relevance Dendritic Cell-Specific ICAM-3-Grabbing Non-Integrin (DC-SIGN; CD209) is a type II plasma membrane PRR abundantly expressed in antigen-presenting cells such as dendritic cells (DCs) and activated macrophages. As member of the C-type Lectin Receptor (CLR) family, DC-SIGN binds a plethora of pathogens, ranging from viruses like HIV-1, ebola virus, and hepatitis C virus, to larger pathogens like Mycobacterium tuberculosis and Candida albicans. This N80A mutant lacks the N-glycosylation ligand binding domain.
Production Details CHO cell lines stably expressing DC-SIGN-N80 were established by Lipofectamin 2000 transfection, and were cultured in Ham’s F-12 medium supplemented with 10% heat-inactivated FBS, 1% Antibiotic Antimycotic Solution , and 0.5 mg/ml of the aminoglycoside antibiotic G418.
Conditional No
Research Area Cell Signaling & Signal Transduction, Immunology
Recommended Growing Conditions Ham’s F-12 medium supplemented with 10% heat-inactivated FBS, 1% Antibiotic Antimycotic Solution , and 0.5 mg/ml of the aminoglycoside antibiotic G418.

References

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References: 1 entry

Te Riet et al. 2017. Sci Rep. 7(1):6713. PMID: 28751750.


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References: 1 entry

Te Riet et al. 2017. Sci Rep. 7(1):6713. PMID: 28751750.


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Inventor Information

Inventors

Alessandra  Cambi

Alessandra Cambi

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