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Mitochondrial complex II mutated SDHD cell line
RECENTLY UPDATED

Invented by Abey Bandara
Invented at Virginia Tech

Info

Catalogue Number 160706
Antigen/Gene or Protein Targets SDHD
Parental Line HEK293
Synonyms HEK293ΔSDHD
Host Human
Disease Keywords Mitochondrial disease; complex II deficiency; Paraganglioma
Model Knock-Out
Relevance The HEK293ΔSDHD cell line expresses a CRISPR/Cas9 mutated nuclear encoded subunit, SDHD, of mitochondrial complex II (succinate dehydrogenase). This cell line has been shown to significantly alter the function of complex II (CII) of the mitochondrial electron transport chain and displays disruptions to SDHD that mimic clinical presentations of CII deficiency. These cells represent a powerful model of metabolic pathologies, not only for mitochondrial disease, but also for understanding the importance of CII in developing novel therapeutics.

Specific disruptions in SDHD have been reported to present clinically as the following disease conditions:
-Complex II deficiency
-Paraganglioma
Production Details The cell line is currently stored at Virginia Tech.
Research Area Cancer, Drug Discovery & Development, Metabolism
Growth/Phenotype Keywords The mutant has been shown to grow much slower than the parental cell line. The doubling time of the mutant is also significantly slower than that of the parental line.
Recommended Growing Conditions DMEM medium supplemented with 10% Fetal Bovine Serum and 1% Penicillin-Streptomycin at 37ºC temperature and 5-6% CO2.
Positive Control HEK293 parental line
Notes CRISPR edited HEK293 cells.

Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here

This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. Ximbio can provide these modified CRISPR-Cas9 cell lines to companies under a label-use only license.

References

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References: 1 entry

Bandara et al. 2021. BMC Mol Cell Biol. 22(1):35. PMID: 34118887.


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References: 1 entry

Bandara et al. 2021. BMC Mol Cell Biol. 22(1):35. PMID: 34118887.


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