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Monoclonal Antibodies

Anti-blood group B antigen IgG [M18] monoclonal antibody

Invented by Tomasz Klaus , Monika Bzowska , Joanna Bereta
Invented at Jagiellonian University
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From Klaus et al Sci Rep. 2016; 6: 30938. Haemagglutination induced by mouse IgG3. (a) The drawing schematically presents cloning of variable fragments into IgG3 and IgG1 frameworks. (b) IgG1 and IgG3 with the same variable fragments were compared in terms of their ability to agglutinate erythrocytes. Antibody concentration in the analysed cell culture media was determined using ELISA. Representative results of three independent experiments. Scale bar –100 μm. (c) Since O10_IgG3 was less efficient in haemagglutination than M18, the experiment presented in b was repeated using higher concentration of purified O10_IgG3 and O10_IgG1 antibodies. Representative results of two independent experiments. Scale bar –200 μm. (d) The EC50 of antibody binding to immobilized type B RBCs. Data points represent mean values obtained for triplicates of analysed samples. The experiment was performed two times. The EC50 of M18_IgG3 was approximately three times lower than the EC50 of O10_IgG3 in both repetitions.
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Image thumbnail for Anti-blood group B antigen IgG [M18] monoclonal antibody
Catalogue Number 160645
Applications ELISA Fn WB
Antigen/Gene or Protein Targets Human blood group B antigen
Reactivity Human
Relevance From Klaus et al. Sci Rep. 2016 Aug 3;6:30938.
Mouse immunoglobulins M (IgMs) that recognize human blood group antigens induce haemagglutination and are used worldwide for diagnostic blood typing. Contrary to the current belief that IgGs are too small to simultaneously bind antigens on two different erythrocytes, we obtained agglutinating mouse IgG3 that recognized antigen B of the human ABO blood group system.
Host Mouse
Immunogen Human red blood cells
Subclass IgG3
Myeloma Used Sp2/0
Strain Balb/c
Notes From Klaus et al. Sci Rep. 2016 Aug 3;6:30938.
Mouse IgG3 is an intriguing isotype that has the ability to form Fc-dependent oligomers. However, F(ab′)2 fragments of the IgG3 were sufficient to agglutinate type B red blood cells; therefore, IgG3-triggered agglutination did not require oligomerization. Molecular modelling indicated that mouse IgG3 has a larger range of Fab arms than other mouse IgG subclasses and that the unique properties of mouse IgG3 are likely due to the structure of its hinge region. With a focus on applications in diagnostics, we compared the stability of IgG3 and two IgMs in formulated blood typing reagents using an accelerated storage approach and differential scanning calorimetry. IgG3 was much more stable than IgMs. Interestingly, the rapid decrease in IgM activity was caused by aggregation of the molecules and a previously unknown posttranslational proteolytic processing of the μ heavy chain. Our data point to mouse IgG3 as a potent diagnostic tool.
Research Area Other

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References: 1 entry

Klaus et al. 2016. Sci Rep. 6:30938. PMID: 27484487.

ELISA Fn WB

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References: 1 entry

Klaus et al. 2016. Sci Rep. 6:30938. PMID: 27484487.

ELISA Fn WB

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Inventor Information

Inventors

Tomasz Klaus

Tomasz Klaus

Monika Bzowska

Monika Bzowska

Joanna Bereta

Joanna Bereta

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Invented at

Jagiellonian University
Jagiellonian University

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