Recombinant, super stable IgM, anti-blood group B antibody
Invented by Tomasz Klaus , Monika Bzowska , Joanna Bereta
Invented at Jagiellonian University
- Datasheet
- References (2)
- Inventor Info
Info
| Catalogue Number | 160644 |
| Applications | ELISA Fn WB |
| Antigen/Gene or Protein Targets | Human blood group B antigen |
| Reactivity | Human |
| Relevance |
Adapted from Klaus et al Sci Rep. 2018 Jan 11;8(1):519. IgM is a multivalent antibody which evolved as a first line defense of adaptive immunity. It consists of heavy and light chains assembled into a complex oligomer. In mouse serum there are two forms of IgM, a full-length and a truncated one. The latter contains μ' chain, which lacks a variable region. Although μ' chain was discovered many years ago, its origin has not yet been elucidated. The inventing PI's results indicate that μ' chain is generated from a full-length heavy chain by non-enzymatic cleavage of the protein backbone. The cleavage occurred specifically after Asn209 and is prevented by mutating this residue into any other amino acid. The process requires the presence of other proteins, preferentially with an acidic isoelectric point, and is facilitated by neutral or alkaline pH. This unique characteristic of the investigated phenomenon distinguishes it from other, already described, Asn-dependent protein reactions. A single IgM molecule is able to bind up to 12 epitopes via its antigen binding fragments (Fabs). The cleavage at Asn209 generates truncated IgM molecules and free Fabs, resulting in a reduced IgM valence and probably affecting IgM functionality in vivo. |
| Host | Mouse |
| Immunogen | Human red blood cells |
| Subclass | IgM |
| Research Area | Other |
| Immunogen UniProt ID | various |
| Recommended Growing Conditions | The recombinant IgM is transiently expressed in HEK293 cells |
| Notes | Anti-blood group B antigen antibodies have applications in medicine, especially medical diagnostics, in particular blood group determination. The commonly used antibody for such work is the parental version of this recombinant antibody. However, being prone to cleaved, especially at elevated temperatures makes the parental version much less desirable than this super stable recombinant variant. |
References: 2 entries
Klaus et al. 2018. Sci Rep. 8(1):519. PMID: 29323348.
ELISA Fn WB
IgM with improved stability and method for obtaining it and applications
ELISA Fn WB
Add a reference
References: 2 entries
Klaus et al. 2018. Sci Rep. 8(1):519. PMID: 29323348.
ELISA Fn WB
IgM with improved stability and method for obtaining it and applications
ELISA Fn WB
Add a reference
Inventor Information
Inventors
|
Tomasz Klaus |
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|
Monika Bzowska |
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Joanna Bereta |
