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Recombinant Antibodies

PtX Anti-SARS-CoV-2 Spike Protein [S1His] recombinant antibody

Invented at Cape Bio Pharms
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Figure 1. Coomassie and WB blot of S1-His protein. Lane 1 = Purified 12.5ug total (SDS coomassie); Lane 2 = Marker; Lane 3 = Crude extract (WB) detected with anti-His at 1/2000 and anti-mouse HRP at 1/2500. Notes: The expression of S1-His was observed at 100kDa (SDS PAGE) and predicted size using CLC is 80kDa. The predicted number of N-glycan sites using NetNGlyc 1.0 server: 13 Predicted size with N-glycans: 106KDa (assuming each glycan gave 2KDa).
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Image thumbnail for PtX Anti-SARS-CoV-2 Spike Protein [S1His] recombinant antibody
Catalogue Number 160595
Applications WB
Antigen/Gene or Protein Targets Receptor Binding domain (RBD) SARS-CoV-2 Spike protein
Synonyms S1
Reactivity Virus
Relevance The spike glycoprotein of SARS-CoV-2 is known to interact with the ACE2 receptor on human respiratory epithelial cells. The receptor binding domain (RBD) is part of the S1 subunit of the spike and is responsible for recognizing the ACE2 receptor.
Host Hybrid
Immunogen Receptor Binding Domain S1 SARS-CoV-2
Subclass IgG1
Formulation pH 0.1M Phosphate Buffered saline, purified using immobilized metal affinitychromatography(IMAC).
Concentration 1mg/ml
Molecular Weight (kDa) 100
Research Area Immunology, Virology
Immunogen UniProt ID P0DTC2 (SPIKE_SARS2)
Notes DNA encoding the receptor binding domain (RBD) portion of the Spike protein of SARS-CoV-2 fused to a polyhistidine and a SEKDEL tag at the C-terminus was recombinantly expressed in Nicotiana benthamiana.
Host species: Mouse, made in N. Benthamiana plants.
Purity 90 -95% as determined by SDS-PAGE. Stability : Up to 1 year from delivery

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Invented at

Cape Bio Pharms
Cape Bio Pharms

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