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Catalogue Number
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160593 |
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Antigen/Gene or Protein Targets
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Exosomes expressing CD63
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Model
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Knock-In
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Relevance
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This mouse models allows for researchers to track an visualize endogenous exosomes in real time. A NanoLuc reporter is fused to the exosome surface marker CD63 and expression is under control of a cardiomyocyte-specific promoter and conditional for tamoxifen.
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Conditional
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Yes
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Conditional Description
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CD63Nan
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Strain
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C57BL/6
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Mouse Genetic Background/Cross History
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In order to achieve a controllable exosome tagging, a dSTOP sequence flanked by two loxP sites was introduced upstream of CD63NanoLuc, and the expression cassette was driven by cardiomyocyte-specific α-MHC promoter (Fig). Linearized DNA containing αMHC-STOP-CD63NanoLuc cassette was used for pronuclear microinjection to generate the founder of the transgenic strain.
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Research Area
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Cardiovascular, Cell Type or Organelle Marker, Cell Signaling & Signal Transduction
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Notes
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Please see linked reference that details the generation and characterization of this mouse model.
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