Erythropoietin expressing D1-MSC cell line
Invented by Jose Luis Pedraz at University of the Basque Country, EHU
GFP-expressing D1-MSC cell line
Invented at University of the Basque Country, EHU
- Datasheet
- References (3)
- Inventor Info
Info
| Catalogue Number | 160552 |
| Antigen/Gene or Protein Targets | GFP |
| Parental Line | D1-MSC |
| Host | Mouse |
| Tissue | Bone Marrow |
| Model | Stem Cells |
| Relevance | Mesenchymal stem cells (MSCs) are multipotent stem cells found in bone marrow that are important for making and repairing skeletal tissues, such as cartilage, bone and the fat found in bone marrow. D1-MSC's are a cell line of murine MSCs from bone marrow. |
| Production Details | Complementary DNA (cDNA) of GFP was cloned from the plasmid pAcGFP1-N1 containing GFP from Aequorea coerulescens and cloned on a lentiviral vector (pSIN) to give pSIN-EF2-GFP-Pur. This was used to transfect HEK293 cells from which the supernatant-containing lentivirus was harvested after 3 days. This supernatant was used to transduce D1-MSCs for 1 month after which GFP expression was confirmed by fluorescence microscopy. Lack of virus production by the now GFP-expressing D1-MSCs was confirmed through attempts at using their supernatant to infect other cells. Insertion of GFP cDNA sequence into the genome was determined by PCR. |
| Conditional | No |
| Research Area | Developmental Biology, Fluorescent Cell Imaging, Other |
| Growth/Phenotype Keywords | Adherent cells |
| Recommended Growing Conditions | Dulbecco´s Modified Eagle´s Medium (DMEM) high glucose media supplemented with 10% heat-non-inactivated fetal calf serum and 1 unit/mL of penicillin-streptomycin and puromycin at 12.5 μg/mL. |
| Notes | D1-MSCs were transduced with the lentiviral construct pSIN-EF2-GFP-Pur, which codifies GFP from Aequorea coerulescens under the human elongation factor-1 alpha (EF-1 alpha) promoter, linked to puromycin resistance through an internal ribosome entry site (IRES) motif. |
Sonic hedgehog-expressing D1-MSC cell line
Invented by Jose Luis Pedraz at University of the Basque Country, EHU
Luciferase-expressing D1-MSC cell line
Invented by Jose Luis Pedraz at University of the Basque Country, EHU
References: 3 entries
Espona-Noguera et al. 2019. Int J Pharm. 560:65-77. PMID: 30742984.
Espona-Noguera et al. 2019. Int J Pharm. 560:65-77. PMID: 30742984.
Megías et al. 2017. Int J Pharm. 518(1-2):270-280. PMID: 28011343.
Add a reference
References: 3 entries
Espona-Noguera et al. 2019. Int J Pharm. 560:65-77. PMID: 30742984.
Espona-Noguera et al. 2019. Int J Pharm. 560:65-77. PMID: 30742984.
Megías et al. 2017. Int J Pharm. 518(1-2):270-280. PMID: 28011343.
Add a reference
