IL-10-expressing Raw 264.7 cell line
Invented by Jose Luis Pedraz , Jesús Ciriza , Laura Saenz del Burgo
Invented at University of the Basque Country, EHU
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Info
| Catalogue Number | 160550 |
| Antigen/Gene or Protein Targets | Interleukin-10 |
| Parental Line | Raw 264.7 |
| Synonyms | IL-10, Interleukin 10 |
| Host | Mouse |
| Disease Keywords | Epithelial to mesenchymal transition |
| Relevance |
Microencapsulated macrophages release conditioned medium able to prevent epithelial to mesenchymal transition. Epithelial to mesenchymal transition (EMT) has emerged as a key process in the development of renal fibrosis. In fact, EMT-derived fibroblasts contribute to the progression of chronic renal disease. In addition, anti-inflammatory M2 macrophages have exhibited a great influence on renal fibrosis. However, because of the high impact that the inputs of different environmental cytokines have on their phenotype, macrophages can easily lose this property. This is why we aimed to modify the Raw 264.7 cell line in order to induce a M2 stable phenotype. M1 and M2 macrophages are main characters in the fibrotic process, chiefly preventing epithelial to mesenchymal transition (EMT). EMT, a process that make fully differentiated epithelial cells undergo transition to a fibroblast phenotype, has emerged as one important pathway in the development of renal fibrosis. Cells in EMT acquire mesenchymal migratory capacity and travel across the disrupted tubular basement membrane into the interstitial microenvironment. EMT-derived fibroblasts within the interstitium contribute to the progression of chronic kidney disease by facilitating deposition of interstitial extracellular matrix. The use of transplanted macrophages, pumping out active factors directly at the site, has proven to be an emergent technology in order to restore kidney function or modulate fibrosis through its role on EMT. However, macrophages, when transplanted in vivo, can lose their beneficial properties, depending on the environments. Thus, to preserve the macrophage regenerative effects, these cells were modified in order to induce and maintain their M2 phenotype. |
| Conditional | Yes |
| Conditional Description | Il-10 is expressed as long as these cells are grown in media containing 10ug/ml of blasticidine. |
| Research Area | Cancer, Dermatology, Extracellular Matrix, Stem Cell Biology |
| Growth/Phenotype Keywords | Adherent cells |
| Recommended Growing Conditions | Cells must be grown in blasticidine containing media (90% RPMI-1640 + 10% h.i. FBS). |
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Inventor Information
Inventors
|
Jose Luis Pedraz |
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|
Jesús Ciriza |
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Laura Saenz del Burgo |
