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Monoclonal Antibodies

Anti-Histone H3 [BT164] monoclonal antibody

Invented by Johan van der Vlag
Invented at Radboud UMC
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From: van Bavel et al January 2011Annals of the rheumatic diseases 70(1):201-7 T164 specifically stains and precipitates H3K27me3. (A) BT164 (green) immunofl uorescence staining co-localises (yellow) only with antibodies against methylated H3K27 (red). No co-localisation is observed with antibodies (red) directed against other possible modifi cations present in the epitope of BT164. Antibody (Ab) #34, which recognises unmodifi ed H3, does not show co-localisation with BT164. (B) BT164 immunoprecipitates apoptotic trimethylated histone H3K27. Apoptotic chromatin immunoprecipitated with BT164 is recognised by BT164, as expected, and by the anti-H3K27me3 antibody, while precipitated chromatin does not contain acetylated H3K27 since no staining is observed with anti-H3K27ac.
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Image thumbnail for Anti-Histone H3 [BT164] monoclonal antibody
Catalogue Number 160535
Applications ELISA IF WB
Antigen/Gene or Protein Targets Histone H3
Reactivity Human and Mouse
Relevance Histone H3 is one of the five main histones involved in the structure of chromatin in eukaryotic cells. H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. This makes them an important element to study in the field of epigenetics.
Host Mouse
Immunogen apoptotic chromatin
Notes T164 specifically stains and precipitates H3K27me3.
Research Area Apoptosis and Programmed Cell Death, Epigenetics & Nuclear Signalling

References

There are 1 reference entries for this reagent.

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References: 1 entry

van Bavel et al. 2011. Ann Rheum Dis. 70(1):201-7. PMID: 20699234.

ELISA IF WB

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References: 1 entry

van Bavel et al. 2011. Ann Rheum Dis. 70(1):201-7. PMID: 20699234.

ELISA IF WB

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Inventor Information

Inventors

Johan van der Vlag

Johan van der Vlag

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Invented at

Radboud UMC
Radboud UMC

18 reagents listed on Ximbio