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Anti-CRF polyclonal antibody (Affinity Purified, Sheep)

Invented at Flinders University

Info

Catalogue Number 160429
Applications ELISA IHC IF
Antigen/Gene or Protein Targets An 18 amino acid synthetic CRF peptide (H-CEQLAQQAHSNRKLMEII-OH), conjugated to Keyhole Limpet Hemocyanin (KLH).
Reactivity Human, Mouse, Rat and Sheep
Host Sheep
Method of Purification Affinity Purified
Notes FORM:
Affinity Purified, Lyophilised

SPECIFICITY:
Specific for CRF. Confirmed by Immunohistochemical analysis of Paraffin Embedded Rat Brain (Hypothalamus).

IMMUNO-HISTOCHEMISTRY:
Use at 1µg/100µl for Immunofluorescence staining and 1µg/ml for DAB Staining on Paraffin Embedded Sections. The optimal dilution should be determined by the end user.

STORAGE:
Long term - 70ºC dry Short term - 20ºC solution or 4ºC with appropriate antibacterial agent. Avoid repeated freezing and thawing. Glycerol (1:1) can be added for additional stability.

PRODUCT PREPARATION:
Reconstitute with addition of 20 µl of sterile water.

KNOWN USES:
ELISA (1:100 to 1:10,000), Immunohistochemistry (1:1000), Immunofluorescence Staining (1:100).
Research Area Fluorescent Cell Imaging, Other, Structural Biology

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