Cancer Research Technology
Log in Register
Menu

SCN 2.2 cell line

Invented at Texas A&M University

Info

Catalogue Number 160400
Antigen/Gene or Protein Targets suprachiasmatic nucleus (rat)
Host Rat
Tissue Brain
Disease Keywords Mental Health Disorders
Model Immortalised Line
Relevance This cell line is derived from rat suprachiasmatic nucleus and are useful in research studying circadian rhythms. This cell line has been characterized by circadian clock properties, distinguishing mammalian SCN in situ. SCN 2.2 displayed extended growth potential without transformed or tumorigenic properties, expresses E1A in all cell nuclei, and heterogenous cell types in various stages of differentiation. SCN 2.2 have glial-like (glial markers were unidentified) and neuronal- like morphologies.
Production Details Primary cultured cells from rat suprachiasmic nucleus were immortalized with retroviral vector encoding adenovirus 12S E1A gene. Cell lines were derived from surviving, drug-resistant colonies propagated without antibiotics on poly-D-lysine or mouse laminin-coated culture dishes in MEM with 10% FBS, 2ug.mL glucose and 292 ug/mL L-glutamine.
Conditional No
Research Area Neurobiology

References

There are 1 reference entries for this reagent.

View All References

References: 1 entry

Earnest et al. 1999. J Neurobiol. 39(1):1-13. PMID: 10213449.


Add a reference

References: 1 entry

Earnest et al. 1999. J Neurobiol. 39(1):1-13. PMID: 10213449.


Add a reference

Inventor Information

No inventors are currently linked to this reagent.

Add an inventor