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Catalogue Number
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160394 |
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Antigen/Gene or Protein Targets
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canine muscle cell line
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Host
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Canine
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Tissue
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Skeletal
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Disease Keywords
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Muscle Disorders, Duschenne muscular dystrophy (DMD), myotubular myopathy, Canine myopathies
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Model
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Immortalised Line
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Relevance
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This Myok9 cell line is derived from skeletal muscle from a genotypically normal dog and has been immortalized to study muscle disorders in canines. This cell line expresses dystrophin and other muscle-specific proteins during differentiation.
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Production Details
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Surgical biopsy of vastus lateralis muscle was performed on a 3-year old normal golden retriever. Myoblast extraction using pre-plate technique was performed. Primary cells were extracted and immunostained for desmin and PAX7. Immortalization was performed with lentivirus encoding SV40 large T antigen with puromycin resistance gene at MOI 2. Cells were selected with 1ug/ml puromycin for 3 days and passaged for 3 cycles.
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Conditional
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No
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Research Area
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Other
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