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YC20 - Protein labelling reagent small molecule (tool compound)

Invented by Jeffrey Keillor
Invented at University of Ottawa

Info

Catalogue Number 159671
Antigen/Gene or Protein Targets Protein of interest to be labelled
Synonyms YC20
Type Fluorescent Probe
Relevance This labeling reagent reacts with sulfhydryl groups on target proteins. By tagging proteins of interest with a di-cysteine peptide tag, (dC-10α), a researcher can use this labeling reagent to detect dC-10α-tagged proteins intracellularly and in real time.
Key Attributes
  • Highly Specific due to dimalemide fluorogen specificity for dicysteines within the dC10α-tag

  • Stable fluorescence intensity labeling over hours of imaging

  • Low perturbation of function and localization of proteins due to smaller size compared to standard fluorescent proteins

  • Adaptable to experimental needs by cloning target sequence dC-10α (dicysteine tag) to protein of interest

  • No washing step required for imaging

  • Non-toxic in tested cell line
  • Research Area Fluorescent Cell Imaging
    Notes Patent: US 9,701,667

    References

    There are 1 reference entries for this reagent.

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    References: 1 entry

    Chen et al. 2014. Angew Chem Int Ed Engl. 53(50):13785-8. PMID: 25314130.


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    References: 1 entry

    Chen et al. 2014. Angew Chem Int Ed Engl. 53(50):13785-8. PMID: 25314130.


    Add a reference

    Inventor Information